Clinical relevance of miR-423-5p levels in chronic obstructive pulmonary disease patients

Abstract

Objective:

This study aimed to examine changes in miRNAs expression profile of COPD patients.

Methods:

Thirty-six COPD patients as well as thirty-three healthy volunteers were recruited. Total RNAs were collected from the plasma of each participant. The differentially expressed miRNAs in COPD were screened from the GEO database. RT-qPCR was carried out to detect miRNA expression.

Results:

In total, 9 out of 55 miRNAs were expressed differentially in COPD patients. Confirmed by RT-qPCR validation, 6 miRNAs increased while 3 miRNAs decreased. Further analysis of miR-423-5p, which has not been reported in COPD, showed that AUC for the diagnosis of COPD was 0.9651, and miR-423-5p levels was inversely correlated with the duration of smoking.

Conclusion:

The present study demonstrates that miR-423-5p is a potential marker for identifying COPD patients.

Keywords:
COPD; miR-423-5p; GEO; Receiver operating curve

HIGHLIGHTS

Relationship of plasma miR-423-5p expression in COPD patients as well as smoking history.

9 miRNAs were dysregulated in COPD patients.

miR-423-5p has potential value as a clinical diagnosis of COPD.