New homologues of the Rpi-chc1 gene in wild and cultivated Solanum species

Figure 1
Results of PCR amplification of total DNA isolated from accessions of wild and cultivated Solanum species with primers specific to the Rpi-chc1 gene. 1 - S. berthaultii К23047, 2 - S. berthaultii К23154, 3 - S. berthaultii К24267, 4 - S. berthaultii К19961, 5 - S. chacoense PI189219, 6 - S. chacoense GLKS 176, 7 - S. chacoense GLKS 1006, 8 - S. chacoense GLKS 135, 9 - S. chacoense CGN 22725, 10 - S. chacoense K19264, 11 - S. maglia К24604, 12 - S. maglia К2883, 13 - S. microdontum CGN 20640, 14 - S. microdontum CGN 23050, 15 - “Bintje”, 16 - “Desiree”, 17 - “Early Rose”, 18 - “Eersteling”, 19 - “Escort”, 20 - “Gloria”, 21 - “Jubel”, 22 - “Nayada”, 23 - “Priekul’skij rannij”, 24 - “Sarpo Axona”, 25 - “Sarpo Mira”, 26 - “Zagadka Pitera”, 27 - hybrid 2372-60, 28 - hybrid 2522-173, 29 - hybrid 2584-7, 30 - hybrid 2585-67, M - 1 kb DNA Ladder.

Figure 2
Phylogenetic tree of nucleotide sequences of the Rpi-chc1 gene variants and homologues thereof in Solanum species. Rpi-chc1.1, Rpi-chc1.2, Rpi-ber1.1, Rpi-ber1.2, Rpi-ber1.3, Rpi-ber1.4, Rpi-tar1.1, Rpi-tar1.3 and Rpi-tub1.3 - previously known variants of the Rpi-chc1 gene in S. chacoense, S. berthaultii, S. tarijense and S. tuberosum (NCBI GenBank accession nos. MW383255, MW410797, MW390806, MW410793, MW410798, MW410803, MW390807, MW410799 and MW410800, respectively). New homologues of the Rpi-chc1 gene found in this study and their NCBI GenBank accession nos. are in bold. Also, variants of the prototype gene Rpi-chc1.1 and Rpi-chc1.2 are highlighted in bold. Bootstrap values are shown near the branches.

Figure 3
LRR domain of the Rpi-chc1 protein. Arrows indicate the location of this domain (the numbers of the initial and final amino acids) relative to the full-length protein. Numbers above the vertical lines indicate the positions of the functionally important amino acids that are discussed in this article. Bold numbers indicate the positions in which previously unknown amino acids were found. Italicized numbers indicate the positions where the amino acids correspond to known variants of the Rpi-chc1 protein. Numbers on the LRR domain itself indicate the LRR numbers in which the abovementioned substitutions are found.