MS INTA 416: A new Argentinean wheat cultivar carrying Fhb1 and Lr47 resistance genes

MS INTA 416 is a hard red winter wheat selected for high yield potential and good bread-making quality, combined with moderate resistance to Fusarium-head-blight and high resistance to leaf-rust, due mainly to presence of resistance genes Fhb1 and Lr47. MS INTA 416 is adapted to main production areas of Central-Argentina.

Key words:
Backcross; marker-assisted selection; yield; bread-making quality; disease control

Authorship

Carlos T. Bainotti

INTA EEA Marcos Juárez, Ruta 12, km 3, (2580) Marcos Juárez, Córdoba, Argentina. INTA EEA Marcos JuárezArgentinaCórdoba, ArgentinaINTA EEA Marcos Juárez, Ruta 12, km 3, (2580) Marcos Juárez, Córdoba, Argentina.

Silvina Lewis

Instituto de Recursos Biológicos, INTA, (1686) Hurlingham, Buenos Aires, Argentina Instituto de Recursos BiológicosArgentinaBuenos Aires, ArgentinaInstituto de Recursos Biológicos, INTA, (1686) Hurlingham, Buenos Aires, Argentina

Pablo Campos

INTA EEA Bordenave, Zona Rural, (8187) Bordenave, Buenos Aires, Argentina INTA EEA BordenaveArgentinaBuenos Aires, ArgentinaINTA EEA Bordenave, Zona Rural, (8187) Bordenave, Buenos Aires, Argentina

Enrique Alberione

Nicolás Salines

Dionisio Gomez

Jorge Fraschina

José Salines

María B. Formica

Guillermo Donaire

Leonardo S. Vanzetti

CONICET, Av. Rivadavia 1917 (C1033AAJ) CABA, Argentina CONICETArgentinaCABA, ArgentinaCONICET, Av. Rivadavia 1917 (C1033AAJ) CABA, Argentina

Lucio Lombardo

María M. Nisi

Martha B. Cuniberti

Leticia Mir

María B. Conde

Marcelo Helguera ^**E-mail: helguera.marcelo@inta.gob.ar

*E-mail: helguera.marcelo@inta.gob.ar

SCIMAGO INSTITUTIONS RANKINGS

INTA EEA Bordenave, Zona Rural, (8187) Bordenave, Buenos Aires, Argentina INTA EEA BordenaveArgentinaBuenos Aires, ArgentinaINTA EEA Bordenave, Zona Rural, (8187) Bordenave, Buenos Aires, Argentina

CONICET, Av. Rivadavia 1917 (C1033AAJ) CABA, Argentina CONICETArgentinaCABA, ArgentinaCONICET, Av. Rivadavia 1917 (C1033AAJ) CABA, Argentina

Figures | Tables

Figures (2)
Tables (4)

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Figure 1
1% agarose gel electrophoresis showing PCR amplification with Lr47-specific primers using genomic DNA from BC6 plants. The Lr47 DNA fragment is indicated by a white arrow. The 500bp fragment of the DNA marker (lane M) is indicated with a red arrow. Lanes 1-4: Lr47-heterozygous plants, 5-6: homozygous negative plants, 7: ProINTA Oasis, 8: Pavon S3 (Lr47 donor).

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Figure 2
PCR profiles of wms493 (A) and wms533 (B) microsatellite markers flanking Fhb1, obtained from BC3F2 plants. For (A), in lane M, (size standard DNA), 200bp and 180bp fragments are indicated. Lane 1: Fhb1 heterozygous plants; lanes 2, 4, 8, 9: Fhb1 homozygous plants; lane 3: R4004; lane 5: Sumai 3 (Fhb1 donor); lanes 6-7: negative homozygous plants. For (B), in lane M, 160bp and 150bp fragments are indicated. Lane1: Fhb1 homozygous plant; lanes 2-7: Fhb1 heterozygous plants; lane 8: R4004; lane 9 Sumai 3 (Fhb1 donor).

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Table 1
Performance of MS INTA 416 and other hard wheat cultivars in Marcos Juárez, Argentina, 2012-2014

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Table 2
Heading date of Argentinian winter wheat cultivars considering eight successive sowing dates (in bold). Data obtained at the experimental station of Marcos Juárez, 2014

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Table 3
Grain yield (kg ha^-1) of MS INTA 416 and controls at 18 trial locations (RYT), in 2012, 2013 and 2014. In bold, grain yields of MS INTA 416 ranked highest or second highest

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Table 4
Seedling stage infection types of wheat cultivars and MS INTA 416 after inoculation with dominant leaf rust and stem rust races in Argentina in 2014

Figure 1 1% agarose gel electrophoresis showing PCR amplification with Lr47-specific primers using genomic DNA from BC6 plants. The Lr47 DNA fragment is indicated by a white arrow. The 500bp fragment of the DNA marker (lane M) is indicated with a red arrow. Lanes 1-4: Lr47-heterozygous plants, 5-6: homozygous negative plants, 7: ProINTA Oasis, 8: Pavon S3 (Lr47 donor).

Figure 2 PCR profiles of wms493 (A) and wms533 (B) microsatellite markers flanking Fhb1, obtained from BC3F2 plants. For (A), in lane M, (size standard DNA), 200bp and 180bp fragments are indicated. Lane 1: Fhb1 heterozygous plants; lanes 2, 4, 8, 9: Fhb1 homozygous plants; lane 3: R4004; lane 5: Sumai 3 (Fhb1 donor); lanes 6-7: negative homozygous plants. For (B), in lane M, 160bp and 150bp fragments are indicated. Lane1: Fhb1 homozygous plant; lanes 2-7: Fhb1 heterozygous plants; lane 8: R4004; lane 9 Sumai 3 (Fhb1 donor).

Table 1 Performance of MS INTA 416 and other hard wheat cultivars in Marcos Juárez, Argentina, 2012-2014

HD = days from emergence to heading; PH = plant height without awns in cm; LR = leaf rust incidence and severity based on modified Cobb scale (Peterson et al. 1948Peterson RF, Campbell AB and Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research 26: 496-500.); FHB = Fusarium head blight incidence and severity based on the scale of Stubbs et al. (1986Stubbs R, Prescott JM, Saari EE, Dubin HJ (1986) Cereal disease methodology manual. CIMMYT, Mexico, 51p.); LS = leaf spot diseases incidence and severity based on the scale of Stubbs et al. (1986); TW = test weight in kg hl-¹; GP = grain protein content in g kg^-1; LV = loaf volume in cm³; W = general gluten strength by the Chopin Alveograph and GW = 1000-grain weight in grams.

Table 2 Heading date of Argentinian winter wheat cultivars considering eight successive sowing dates (in bold). Data obtained at the experimental station of Marcos Juárez, 2014

dnf: did not flower

Table 3 Grain yield (kg ha^-1) of MS INTA 416 and controls at 18 trial locations (RYT), in 2012, 2013 and 2014. In bold, grain yields of MS INTA 416 ranked highest or second highest

Table 4 Seedling stage infection types of wheat cultivars and MS INTA 416 after inoculation with dominant leaf rust and stem rust races in Argentina in 2014

† '0'= no visible uredia; ';'= hypersensitive flecks; '1'= small uredia with necrosis; '2'= small to moderate-sized uredia with green islands, surrounded by necrosis or chlorosis; '3'= moderate uredia size, with or without chlorosis; '4'= large uredia without chlorosis; 'N'= necrosis. Symbols '-' and '+' indicate smaller or larger uredinia. The most common infection is listed first. For example, 11+; indicates infection types 1 and 1+.

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Crop Breeding and Applied Biotechnology Universidade Federal de Viçosa, Departamento de Fitotecnia, 36570-000 Viçosa - Minas Gerais/Brasil, Tel.: (55 31)3899-2611, Fax: (55 31)3899-2611 - Viçosa - MG - Brazil
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MS INTA 416: A new Argentinean wheat cultivar carrying Fhb1 and Lr47 resistance genes

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[1] *E-mail: helguera.marcelo@inta.gob.ar