Recurrent selection in common bean aiming at resistance to white mold in a greenhouse

Lopes, Fernanda Souza; Leite, Monik Evelin; Porto, Antonio Carlos Mota; Miguel, Luciana Aparecida; Reis, Vanessa de Oliveira Lima; Santos, João Bosco dos

doi:10.1590/1984-70332019v19n1a13

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Crop Breeding and Applied Biotechnology

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ARTICLE • Crop Breed. Appl. Biotechnol. 19 (01) • Jan-Mar 2019 • https://doi.org/10.1590/1984-70332019v19n1a13 copy

Recurrent selection in common bean aiming at resistance to white mold in a greenhouse

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The objectives of this study were to estimate the genetic progress of mass selection for white mold resistance in common bean, evaluated in a greenhouse in cycle XII of recurrent selection (900 S₀ plants), and compare it with field selection in previous cycles. In addition, progress was compared with microsatellite marker-assisted selection (MAS) among S_0:1 progenies. The 79 most resistant S_0:1 were evaluated under field conditions using a 9×9 simple lattice design; the 21 best S_0:2 and those selected from cycles IX, X, and XI were evaluated in a 6×6 triple lattice. Genetic progress was 4.25% per cycle, and 12.17% between cycles XI and XII, showing higher selection efficiency in the greenhouse. The phenotypic gain and gain from assisted selection among the S_0:1 progenies were 5.08 and 1.57%, respectively, and the low value of MAS was due to only two markers (BM189 and BMD20) explaining the resistance.

Keywords:
Phaseolus vulgaris L.; Sclerotinia sclerotiorum; gain from selection; phenotypic selection; QTL; marker-assisted selection

Cultivar/Strain	Type/Weight 100 grains (g)	Growth habit
1-RP-2	Carioca/25	II
2-MA-IV-18-266	Carioca/23	II
3-BRS - Cometa	Carioca/23	II
4-VC-16	Carioca/25	III
5-BRSMG - Majestoso	Carioca/25	III
6-CNFRJ10564	Pintado/42	I
7-ESAL 550	Jalo/45	III
8- BRSMG - Talismã	Carioca/22	II
9-RC2-G122-67	Carioca/25	II
10-RC2-G122-72	Carioca/23	II
11-RC1-ExRico-26	Carioca/23	II
12-RC1-ExRico-97	Carioca/20	II
13-A195**	Bege/54	I
14-RCII M20 xG122*	Carioca/24	II
15-OPNS x VC3-41*	Carioca	III
16-EMB9*	Carioca	II
17- CNFC10722*	Carioca	II
18- BRS Vereda*	Rosinha/26	II

Primer	Source	Primer	Source
PV-gaat001	(Yu et al. 2000Yu K, Park SJ, Poysa V and Gepts P (2000) Integrating of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). Journal of Heredity 91: 429-434)	ATA9	(Antonio et al. 2012Antonio RP, Santos JB, Alves FC, Gonçalves PRC and Lara LAC (2012) Seleção assistida por marcadores de DNA em retrocruzamento visando resistência ao mofo branco em feijoeiro. Revista Caatinga 25: 61-67.)
GATS91	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	BMc5	(Díaz and Blair 2006Díaz LM and Blair MW (2006) Race structure within the Mesoamerican gene pool of common bean (Phaseolus vulgarisL.) as determined by microsatellite markers. Theoretical and Applied Genetics 114: 143-154.)
BM156	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	IAC 07	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BM172	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	IAC 27	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BM175	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	IAC 37	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BM184	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	IAC 45	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BM189	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	IAC 51	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BM197	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)	IAC 63	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BMD15	(Blair et al. 2003Blair MW, Pedraza F, Buendia HF, Gaitán-Solís E, Beebe SE, Gepts P and Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 107: 1362-1374.)	IAC 71	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
BMD20	(Blair et al. 2003Blair MW, Pedraza F, Buendia HF, Gaitán-Solís E, Beebe SE, Gepts P and Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 107: 1362-1374.)	IAC 74	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
PVBR189	(Souza et al. 2016Souza DA, Balestre M, Pamplona AKA, Leite ME, Dias JA and Santos JB (2016) White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population. Genetics and Molecular Research 15: 1124-1135.)	IAC 77	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
PVBR93	(Grisi et al. 2007Grisi MCM, Blair MW, Gepts P, Brondani C, Pereira PAA and Brondani RPV (2007) Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558. Genetics Molecular Research 6: 691-706.)	IAC 98	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
PVESTBR221	(Garcia et al. 2011Garcia RAV, Rangel PN, Brondani C, Martins WS, Melo LC, Carneiro MS, Borba TCO and Brondani RPV (2011) The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris. BMC Genetics 12: 1-7.)	IAC 117	(Benchimol et al. 2007Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007) Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of mesoamerican and Andean origins using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762.)
PVESTBR279	(Garcia et al. 2011Garcia RAV, Rangel PN, Brondani C, Martins WS, Melo LC, Carneiro MS, Borba TCO and Brondani RPV (2011) The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris. BMC Genetics 12: 1-7.)	PVBR 21	(Buso et al. 2006Buso GSC, Amaral ZPS, Brondani RPV and Ferreira ME (2006) Microsatellite markers for the common bean - Phaseolus vulgaris. Molecular Ecology Notes 6: 252-254.)
PVESTBR42	(Lara et al. 2015Lara LAC, Santos JB, Balestre M, Lima IA, Pamplona AKA, Veloso JS and Silva PH (2015) Identification of QTLs of resistance to white mold in common bean from multiple markers by using Bayesian analysis. Genetics and Molecular Research 4: 1124-1135.)	PVBR 23	(Buso et al. 2006Buso GSC, Amaral ZPS, Brondani RPV and Ferreira ME (2006) Microsatellite markers for the common bean - Phaseolus vulgaris. Molecular Ecology Notes 6: 252-254.)
PVESTBR73	(Souza et al. 2016Souza DA, Balestre M, Pamplona AKA, Leite ME, Dias JA and Santos JB (2016) White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population. Genetics and Molecular Research 15: 1124-1135.)	PVBR 78	(Grisi et al. 2007Grisi MCM, Blair MW, Gepts P, Brondani C, Pereira PAA and Brondani RPV (2007) Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558. Genetics Molecular Research 6: 691-706.)
SSR-IAC134	(Cardoso et al. 2008Cardoso JMK, Oblessuc PR, Campos T, Sforça DA, Carbonell SAM, Chioratto AF, Formighieri EF, Souza AP and Benchimol LL (2008) New microsatellite markers developed from an enriched microsatellite common bean library. Pesquisa Agropecuária Brasileira 43: 929-936.)	PVag003	(Yu et al. 2000Yu K, Park SJ, Poysa V and Gepts P (2000) Integrating of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). Journal of Heredity 91: 429-434)
SSR-IAC159	(Cardoso et al. 2008Cardoso JMK, Oblessuc PR, Campos T, Sforça DA, Carbonell SAM, Chioratto AF, Formighieri EF, Souza AP and Benchimol LL (2008) New microsatellite markers developed from an enriched microsatellite common bean library. Pesquisa Agropecuária Brasileira 43: 929-936.)	BMC 222	(Blair et al. 2009Blair MW, Torres MM, Giraldo MC and Pedraza F (2009) Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgarisL.). BMC Plant Biology 9: 100. )
ATA7	(Antonio et al. 2012Antonio RP, Santos JB, Alves FC, Gonçalves PRC and Lara LAC (2012) Seleção assistida por marcadores de DNA em retrocruzamento visando resistência ao mofo branco em feijoeiro. Revista Caatinga 25: 61-67.)	BM 142	(Gaitán-Solís et al. 2002Gaitán-Solís E, Duque MC, Edwards KJ and Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Science 42: 2128-2136.)

Sources of variation	df	MS	Fc
Rep	2	1.224	8.83*
Block (Rep)	15	0.102	0.73
Group (Cycles + Control)	4	1.402	10.11*
Cycles	3	0.154	1.11
Control vs Cycles	1	4.658	33.59*
Progenies (P)	31	0.496	3.58*
P (C-IX)	2	0.102	0.74
P (C-X)	4	0.037	0.27
P (C-XI)	4	0.216	1.55
P (C-XII)	20	0.422	3.04*
Control	1	5.704	41.13*
Error	54	0.139
Mean progenies CXII		3.012
CV (%)		12.36
Accuracy (%)		84.89
Gain per cycle		-0.215 (6.67%)
R² adjusted		0.9354

Cycle	Progeny	Mean	Cycle	Progeny	Mean
	IPR Corujinha (T)	5.11 a	CXII	56\8	2.97 c
CXII	63\8	3.88 b	CXI	11\261	2.93 c
CXII	62\9	3.60 c	CIX	26\15	2.93 c
CXII	64\1	3.52 c	CX	10\107	2.91 c
CIX	23\7	3.41 c	CXI	11\364	2.91 c
CXII	61\12	3.41 c	CXII	64\14	2.90 c
CXII	59\6	3.36 c	CXII	59\4	2.90 c
CXII	63\2	3.28 c	CXII	55\2	2.89 c
CIX	34\3	3.25 c	CXII	64\5	2.85 c
CXII	56\7	4.19 c	CXII	53\10	2.81 c
CX	10\19	3.19 c	CXII	59\8	2.78 c
CXII	64\9	3.16 c	CX	10\267	2.73 c
CXII	61\10	3.15 c	CXII	59\9	2.70 c
CX	10\34	3.10 c	CXII	53\3	2.61 c
	Cornell 605 (T)	3.09 c	CXII	61\3	2.50 c
CXI	11\121	3.06 c	CXII	51\4	2.49 c
CXI	11\296	3.05 c	CXII	56\5	2.40 c
CX	10\185	3.00 c	CXI	11\185	2.36 c

Genetic and phenotypic parameters
Heritability of S_0:1 progenies (%)	31.16
R² adjusted (multiple regression) (%)	16.81
Mean of the 79 S_0:1 progenies	3.18
Mean of the 16 best S_0:1 progenies	2.66
Mean of the 16 progenies derived from the MAS	3.02
Gain from phenotypic selection	-0.1615 (5.08%)
Gain from marker-assisted selection	-0.05 (1.57%)
Efficiency of marker-assisted selection in relation to phenotypic selection	31.27%

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