PERFORMANCE OF YIELD AND YIELD CONTRIBUTING CHARACTERISTICS OF BC2F3 POPULATION WITH ADDITION OF BLAST RESISTANT GENE

The study was carried out in the University Putra Malaysia (UPM) Rice Research Centre to evaluate the yield performance of newly developed selected blast resistant plants of BC₂F₃ generations derived from a cross between MR263, a high yielding rice variety but blast susceptible and Pongsu Seribu 1, donor with blast resistant (Pi-7(t) and Pi-d (t)1, Pir2-3(t) genes and qLN2 QTL), Malaysian local variety. On the basis of assessed traits, the plants 12, 6, 7, 5, 21, 22, 5, 26, 11, 8, 10, 13 and 15 had the higher yield, blast resistant and good morphological traits. More than 70% heritability was found in days to maturity, plant height, tiller numbers per hill, and panicle per hill, 80% heritability was found in filled grain and yield per hill and more than 90% heritability was found in grain length, grain width and seed weight. Cluster analysis based on the traits grouped 30 plants along with MR263 into seven clusters. According to PCA, the first four principal components account for about 69.3% total variation for all measured traits and exhibited high correlation among the characteristics analyzed.

Index terms:
Yield; rice; fungi resistance

Authorship

Muhammad Mahmudul Hasan

University Putra Malaysia, Faculty of Agriculture, Department of Crop Science, 43400, UPM Serdang, Selangor, MalaysiaUniversity Putra MalaysiaMalaysiaSelangor, MalaysiaUniversity Putra Malaysia, Faculty of Agriculture, Department of Crop Science, 43400, UPM Serdang, Selangor, Malaysia

Mohd Rafii Yusop ^*Autor para correspondência: Mohd Rafii Yusop email: mrafii@upm.edu.my

University Putra Malaysia, Institute of Tropical Agriculture, 43400, UPM Serdang, Selangor, Malaysia, email: mrafii@upm.edu.myUniversity Putra MalaysiaMalaysiaSelangor, MalaysiaUniversity Putra Malaysia, Institute of Tropical Agriculture, 43400, UPM Serdang, Selangor, Malaysia, email: mrafii@upm.edu.my

Mohd Razi Ismail

Maziah Mahmood

University Putra Malaysia, Deparment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, 43400, UPM Serdang, Selangor, MalaysiaUniversity Putra MalaysiaMalaysiaSelangor, MalaysiaUniversity Putra Malaysia, Deparment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, 43400, UPM Serdang, Selangor, Malaysia

Harun Abdul Rahim

Malaysian Nuclear Agency, Agrotechnology and Bioscience Division, Bangi, 43000 Kajang, Selangor, MalaysiaMalaysian Nuclear AgencyMalaysiaSelangor, MalaysiaMalaysian Nuclear Agency, Agrotechnology and Bioscience Division, Bangi, 43000 Kajang, Selangor, Malaysia

Mohammad Abdul Latif

Bangladesh Rice Research Institute, Gazipur-1701, BangladeshBangladesh Rice Research InstituteBangladeshGazipur, BangladeshBangladesh Rice Research Institute, Gazipur-1701, Bangladesh

Autor para correspondência: Mohd Rafii Yusop email: mrafii@upm.edu.my

SCIMAGO INSTITUTIONS RANKINGS

Bangladesh Rice Research Institute, Gazipur-1701, BangladeshBangladesh Rice Research InstituteBangladeshGazipur, BangladeshBangladesh Rice Research Institute, Gazipur-1701, Bangladesh

Figures | Tables

Figures (7)
Tables (5)

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Figure 1:
Schematic representation of development of blast resistant rice variety through marker assisted backcrossing (MABC).

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Figure 2:
Selection of homozygous plants carrying blast resistant gene same as donor allele of Pongsu Seribu 1 (PS1) at BC₂F₂ population using gene linked marker RM263.

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Figure 3:
Selection of homozygous plants carrying blast resistant gene same as donor allele of Pongsu Seribu 1 at BC₂F₂ population using gene linked marker RM5961.

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Figure 4:
Marker assisted foreground selection at BC₂F₃ families for genes using gene linked marker RM5961. MR263- recurrent parent; PS1- Pongsu Seribu 1 (blast resistant parent) and M-50bp ladder.

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Figure 5:
Marker assisted foreground selection at BC₂F₃ families for genes using gene linked marker RM263. MR263- recurrent parent; PS1- Pongsu Seribu (blast resistant parent) and M- 50bp ladder.

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Figure 6:
The dendrogram of 30 blast resistant plants with MR263 based on 10 measured yield and yield contributing characteristics.

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Figure 7:
The 30 blast resistant plants with MR263 based on 10 morphological characteristics.

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Table 1:
Yield and yield contributing characteristics of 30 selected homozygous blast resistant plants.

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Table 2:
Estimation of genetic variables of 10 morphological characteristics of 30 selected homozygous blast resistant plants.

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Table 3:
Groups of 30 blast resistant plants with MR263 based on 10 yield and yield according to cluster analysis.

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Table 4:
Mean values of 10 morphological characters for seven groups revealed by cluster analysis on 30 blast resistant plants with MR263 variety.

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Table 5:
Eigenvectors and eigenvalues of the first four principal components.

Figure 1: Schematic representation of development of blast resistant rice variety through marker assisted backcrossing (MABC).

Figure 2: Selection of homozygous plants carrying blast resistant gene same as donor allele of Pongsu Seribu 1 (PS1) at BC₂F₂ population using gene linked marker RM263.

Figure 3: Selection of homozygous plants carrying blast resistant gene same as donor allele of Pongsu Seribu 1 at BC₂F₂ population using gene linked marker RM5961.

Figure 4: Marker assisted foreground selection at BC₂F₃ families for genes using gene linked marker RM5961. MR263- recurrent parent; PS1- Pongsu Seribu 1 (blast resistant parent) and M-50bp ladder.

Figure 5: Marker assisted foreground selection at BC₂F₃ families for genes using gene linked marker RM263. MR263- recurrent parent; PS1- Pongsu Seribu (blast resistant parent) and M- 50bp ladder.

Figure 6: The dendrogram of 30 blast resistant plants with MR263 based on 10 measured yield and yield contributing characteristics.

Figure 7: The 30 blast resistant plants with MR263 based on 10 morphological characteristics.

Table 1: Yield and yield contributing characteristics of 30 selected homozygous blast resistant plants.

Progeny	Days to maturity	Plant Height (cm)	Tiller number/hill	Panicles /hill	Panicle length (cm)	Filled grain (%)	Grain length	Grain width	Yield /hill (g)	100 seed wt (g)
MR263	103a-c	66.74a-d	28.8a-d	32.2ab	25.77a-d	70a-c	10.22a	2.1a	61.39ab	2.63a
P1	99.4bc	61.98d	28.6a-d	28.4a-c	22.48cd	72.2a-c	10.21a	2.1a	54.67a-c	2.62a
P2	100bc	64.14a-d	25.8a-d	25.8a-c	24.03a-d	62a-c	10.22a	2.1a	53.92a-c	2.65a
P3	96.8c	62.74cd	27.8a-d	27.6a-c	20.57cd	63.4a-c	10.21a	2.1a	51.35a-c	2.65a
P4	101.2a-c	63.24b-d	25.2a-d	25.2a-c	22.55cd	67.6a-c	10.21a	2.1a	51.4a-c	2.64a
P5	99bc	67.58a-d	25.2a-d	25.2a-c	21.86cd	74.2a-c	10.22a	2.1a	57.24a-c	2.63a
P6	102.8a-c	65.9a-d	27.2a-d	27.2a-c	24.58a-d	58.2c	10.21a	2.09a	59.27a-c	2.64a
P7	104.4a-c	72.26a-d	33.6a-c	32ab	22.69b-d	67.8a-c	10.22a	2.09a	56.04a-c	2.64a
P8	103.2a-c	68.06a-d	23cd	23bc	25.29a-d	70.8a-c	10.21a	2.09a	57.1a-c	2.62a
P9	98.4bc	65.54a-d	34.2a-c	33ab	25.42a-d	65a-c	10.21a	2.09a	53.79a-c	2.61a
P10	105.8ab	69.74a-d	28.4a-d	28a-c	25.41a-d	73a-c	10.22a	2.09a	51.04a-c	2.65a
P11	109.4a	62.5d	31.4a-d	30.6a-c	24.41a-d	72.6a-c	10.22a	2.09a	55.82a-c	2.57a
P12	100.6ac	63.88a-d	36.4a	35.2a	23.22a-d	71.4a-c	10.22a	2.1a	62a	2.58a
P13	98.4bc	66.5a-d	29.6a-d	29.2a-c	19.12d	70.6a-c	10.21a	2.1a	55.68a-c	2.62a
P14	97.2bc	65.36a-d	33.4a-c	33ab	22.6b-d	66a-c	10.21a	2.1a	51.24a-c	2.65a
P15	101.00a-c	66.4a-d	32.2a-d	32ab	23.7a-d	68.4a-c	10.22a	2.1a	54.84a-c	2.63a
P16	101.4a-c	64.82a-d	20d	20c	22.97a-d	68.8a-c	10.22a	2.09a	48.25c	2.62a
P17	103.8a-c	67.34a-d	26.4a-d	26.2a-c	21.99cd	74a-c	10.21a	2.09a	49.6bc	2.18a
P18	104.4a-c	63.88a-d	29.2a-d	28.8a-c	23.9a-d	69a-c	10.22a	2.09a	53.41a-c	2.61a
P19	100.4bc	68.3a-d	35.6ab	33.8ab	21.84cd	71.8a-c	10.21a	2.1a	56.65a-c	2.58a
P20	101.00a-c	65.36a-d	27.6a-d	27.6a-c	25.5a-d	59.6bc	10.21a	2.1a	53.76a-c	2.59a
P21	100.6a-c	78.28a	29.2a-d	28.2a-c	22.51cd	77.8a	10.22a	2.1a	57.78a	2.64a
P22	99bc	64.14a-d	28.2a-d	27.8a-c	23.88a-d	66.8a-c	10.22a	2.1a	53.1a-c	2.62a
P23	101.2a-c	67.34a-d	31a-d	30.2a-c	29.9ab	75ab	10.22a	2.1a	51a-c	2.61a
P24	100.6a-c	66.82a-d	26.4a-d	25.4a-c	20.68cd	68.2a-c	10.21a	2.09a	52.84a-c	2.61a
P25	98.4bc	67.12a-d	31a-d	30.4a-c	25.57a-d	67.6a-c	10.22a	2.09a	51.16a-c	2.62a
P26	97.2bc	64.3a-d	28a-d	28a-c	25.14a-d	74.2a-c	10.21a	2.1a	54.93a-c	2.63a
P27	101.00a-c	66.28a-d	27.8a-d	27.8a-c	24.75a-d	77.8a	10.22a	2.09a	49.79bc	2.65a
P28	101.4a-c	77.62ab	23.8cd	23.8bc	30.44a	72.2a-c	10.22a	2.09a	53.43a-c	2.61a
P29	103.8a-c	59.1d	29.2a-d	28.8a-c	22.86b-d	63.4a-c	10.22a	2.1a	50.16a-c	2.65a
P30	101.4a-c	77.37a-c	24.8a-d	27a-c	26.77a-c	73.4a-c	10.21a	2.09a	52.22a-c	2.62a
Mean	101.16	65.89	28.8	28.43	23.95	69.44	10.21	2.1	54.01	2.62
CV%	3.56	9.093	17.88	15.87	12.62	9.47	0.065	0.395	9.07	2.33

Means with different lower case letters are significantly different at P<0.05.

Table 2: Estimation of genetic variables of 10 morphological characteristics of 30 selected homozygous blast resistant plants.

Traits	Mean	MSG	MSE	σ² _G	σ² _P	PCV (%)	GCV (%)	h_B ² (%)	GA (%)
Days to maturity	101.16	57.22	9.006	24.107	33.113	5.6884	4.85359	72.80	8.53105
Plant height	66.8027	97.18272	11.9	42.6414	54.5414	11.0553	9.77511	78.18	17.805
Tiller number/Hill	28.8	81.10666	12.543	34.2818	46.8248	23.76	20.3301	73.21	35.8344
Panicles/Hill	28.43	76.46	13.3754	31.5423	44.9177	23.5739	19.7547	70.22	34.1016
Panicle length (cm)	23.95	38.847	7.14	15.8535	22.9935	20.0215	16.6248	68.94	28.437
Filled grain	69.44	127.66	13.314	57.173	70.487	12.0905	10.8889	81.11	20.202
Grain length(mm)	10.21	0.000436	0.000018	0.00021	0.00023	0.14757	0.14159	92.07	0.27988
Grain width (mm)	2.1	0.000358	0.000008	0.00018	0.00018	0.64418	0.62994	95.62	1.269
Yield/ hill (gm)	54.01	91.489	9.004	41.2425	50.2465	13.1244	11.8905	82.08	22.1914
100 seed weight	2.62	0.00557	0.00014	0.00272	0.00286	2.0394	1.98877	95.09	3.99515

Notes: MSG - Genotype mean squares; MSE - Error mean squares; σ² _G- genetic variance; σ² _P - Phenotypic variance; PCV- Phenotypic coefficient of variation; GCV-Genetic coefficient of variation; h_B ²- Broad-sense heritability; GA- Genetic advanced.

Table 3: Groups of 30 blast resistant plants with MR263 based on 10 yield and yield according to cluster analysis.

Groups	Plants
Cluster I	MR263, P18, P7, P11
Cluster II	P1, P26, P13, P3, P4, P5, P2, P22, P29, P6, P20, P8, P24
Cluster III	P9, P14, P12, P15, P19
Cluster IV	P10, P27, P16, P25, P23
Cluster V	P21
Cluster VI	P28, P30
Cluster VII	P17

Table 4: Mean values of 10 morphological characters for seven groups revealed by cluster analysis on 30 blast resistant plants with MR263 variety.

Groups	Days of maturity	Plant Height (cm)	Tiller number/hill	Panicles /hill	Panicle length (cm)	Filled grain (%)	Grain length	Grain width	Yield /hill (g)	100 seed wt (g)
Cluster I	105.30	66.35	34.36	33.40	24.19	69.85	10.22	2.09	58.81	2.64
Cluster II	100.33	64.45	26.85	26.67	23.29	66.72	10.21	2.10	54.15	2.63
Cluster III	99.52	65.90	31.70	30.90	23.36	68.52	10.21	2.10	53.99	2.61
Cluster IV	101.56	67.06	29.48	28.92	25.72	72.44	10.22	2.09	50.25	2.63
Cluster V	100.60	78.28	20.00	20.00	22.51	77.80	10.22	2.10	57.78	2.61
Cluster VI	101.40	77.50	24.30	25.40	28.61	72.80	10.22	2.09	52.83	2.62
Cluster VII	103.80	67.34	26.40	26.20	21.99	74.00	10.21	2.09	49.60	2.18

Table 5: Eigenvectors and eigenvalues of the first four principal components.

Variables	PCA1	PCA2	PCA3	PCA4
Eigenvalue	2.5568	1.8401	1.4084	1.1182
Variation%	25.6	18.4	14.1	11.2
Cumulative%	25.6	44	58.1	69.2
Days to maturity	0.226	-0.47	-0.2	-0.445
Plant Height(cm)	0.455	-0.032	0.156	0.204
Tiller number	-0.478	-0.423	-0.056	0.136
Panicles /hill	-0.476	-0.418	-0.044	0.155
Panicle length (cm)	0.26	-0.333	0.234	0.203
Filled grain (%)	0.311	-0.134	-0.018	0.498
Grain length	0.12	-0.444	0.359	0.128
Grain width	-0.311	0.3	0.351	0.22
Yield /hill(gm)	0.026	-0.08	0.358	-0.598
100 seed wt(gm)	-0.102	0.039	0.703	-0.077

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PERFORMANCE OF YIELD AND YIELD CONTRIBUTING CHARACTERISTICS OF BC2F3 POPULATION WITH ADDITION OF BLAST RESISTANT GENE

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[1] Autor para correspondência: Mohd Rafii Yusop email: mrafii@upm.edu.my

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PERFORMANCE OF YIELD AND YIELD CONTRIBUTING CHARACTERISTICS OF BC2F3 POPULATION WITH ADDITION OF BLAST RESISTANT GENE

Desempenho produtivo e características de produção de populações de BC2F3 com gene de resistência a brunose

ABSTRACT

PERFORMANCE OF YIELD AND YIELD CONTRIBUTING CHARACTERISTICS OF BC₂F₃ POPULATION WITH ADDITION OF BLAST RESISTANT GENE

Desempenho produtivo e características de produção de populações de BC₂F₃ com gene de resistência a brunose