Melhoramento de uma linhagem de alto rendimento para cultivo comercial por cruzamento de <i>Pholiota adiposa</i>

Pholiota adiposa é um cogumelo com excelentes propriedades nutricionais e medicinais. No entanto, o rendimento do corpo de frutificação deste é baixo e o potencial de cultivo comercial deste fungo é limitado. 279 linhagens cruzadas foram obtidas pelo cruzamento mono-mono de linhagens monocarióticas derivadas de P. adiposa HS5 e HS4. Enzimas ligninolíticas e taxas de crescimento micelial foram utilizadas como marcadores para a triagem das linhagens cruzadas, e 18 linhagens foram seleccionadas para análise posterior. A linhagem cruzada A10B4 apresentou o maior rendimento (ie 165,91 ± 12,56 g por sacola), o qual 31,34 g e, 74,48 g a mais do que as linhagens HS5 e HS4, respetivamente. O tempo de colonização micelial de A10B4 foi 25,18 ± 1,33 d, sendo 5,64 d menor que HS5. A10B4 foi caracterizado, por marcadores moleculares de repetição de sequência inter-simples. Foram observadas diferenças nos produtos de PCR de linhagens parentais e cruzadas. Portanto, a linhagem híbrida recém-desenvolvida A10B4 (Renominada P. adiposa HS54), exibindo alto rendimento, pode ser adequada para cultivo comercial.

Termos para indexação:
Cogumelo; enzimas ligninolíticas; crescimento micelial.

Autoria

Chengbo Rong

Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, ChinaBeijing Academy of Agriculture and Forestry SciencesChinaChinaInstitute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, China

http://orcid.org/0000-0003-3784-0591

Shuang Song

http://orcid.org/0000-0001-5387-5941

Li Yang

http://orcid.org/0000-0003-3258-8497

Xuejiao Pan

http://orcid.org/0000-0002-4820-8033

Yu Liu

http://orcid.org/0000-0002-5429-1910

Shouxian Wang ^**Corresponding author: wangshouxian2002@163.com

http://orcid.org/0000-0001-7035-5497

^*Corresponding author: wangshouxian2002@163.com

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas

Figuras (3)
Tabelas (2)

Thumbnail

Figure 1:
Decolorization of HS4 and HS5 monokaryons on PDA plates containing RBBR. The RBBR decolorization degree was scored as 1 (+), 2 (++), 3 (+++), or 4 (++++). The red circles represent halos of RBBR decolorization. A, B, C, and D show RBBR decolorization degrees of 1, 2, 3, and 4, respectively; 1 represents the lowest degree of RBBR decolorization (The color of the halos is the darkest) and 4 represents the highest degree of RBBR decolorization (The color of the halos is the lightest).

Thumbnail

Figure 2:
Morphological characteristics of HS4, HS5, and crossbred strain fruiting bodies. HS4 and HS5 were parental strains.

Thumbnail

Figure 3:
Amplification products obtained by PCR using ISSR primers and DNA from HS4, HS5, and A10B4. M, marker; lane 1, HS4; lane 2, HS5; lane 3, A10B4. Arrows indicate genetic markers showing a difference between strain A10B4 and the parental strains.

Thumbnail

Table 1:
Mycelial growth rate and decolorization degree of crossbred and parental strains.

Thumbnail

Table 2:
Comparison of the production yields and mycelial colonization times of fruiting bodies.

Figure 1: Decolorization of HS4 and HS5 monokaryons on PDA plates containing RBBR. The RBBR decolorization degree was scored as 1 (+), 2 (++), 3 (+++), or 4 (++++). The red circles represent halos of RBBR decolorization. A, B, C, and D show RBBR decolorization degrees of 1, 2, 3, and 4, respectively; 1 represents the lowest degree of RBBR decolorization (The color of the halos is the darkest) and 4 represents the highest degree of RBBR decolorization (The color of the halos is the lightest).

Figure 2: Morphological characteristics of HS4, HS5, and crossbred strain fruiting bodies. HS4 and HS5 were parental strains.

Figure 3: Amplification products obtained by PCR using ISSR primers and DNA from HS4, HS5, and A10B4. M, marker; lane 1, HS4; lane 2, HS5; lane 3, A10B4. Arrows indicate genetic markers showing a difference between strain A10B4 and the parental strains.

Table 1: Mycelial growth rate and decolorization degree of crossbred and parental strains.

Number	Crossbred strain	Mycelial growth rate (mm/d)	Decolorization degree
Parental strain strain strains strain	HS4	3.46±0.10	1
Parental strain	HS5	2.29±0.17	2
1	A5B1	3.14±0.09	1
2	A1B18	2.81±0.16	2
3	A1B17	2.66±0.23	2
4	A1B11	2.57±0.12	2
5	A1B12	2.57±0.18	2
6	A1B6	2.53±0.12	2
7	A10B5	2.47±0.05	3
8	A16B3	2.46±0.05	3
9	A20B3	2.44±0.08	3
10	A13B4	2.43±0.03	2
11	A8B6	2.40±0.12	3
12	A17B21	2.38±0.06	2
13	A21B6	2.38±0.06	2
14	A4B6	2.36±0.12	3
15	A14B4	2.36±0.15	3
16	A10B4	2.35±0.03	3
17	A20B12	2.35±0.03	3
18	A8B9	2.34±0.08	2
19	A6B6	2.34±0.04	3
20	A13B6	2.31±0.08	2
21	A7B6	2.31±0.03	3
22	A10B12	2.30±0.08	4
23	A16B6	2.30±0.05	4
24	A11B2	2.29±0.06	3
25	A14B21	2.29±0.04	3
26	A4B5	2.27±0.07	3
27	A21B12	2.27±0.05	4
28	A13B21	2.26±0.07	3
29	A10B10	2.25±0.06	3
30	A20B21	2.25±0.03	3
31	A14B6	2.24±0.10	4
32	A4B9	2.20±0.04	3
33	A13B12	2.19±0.03	4
34	A19B12	2.16±0.06	4
35	A12B3	2.16±0.15	4
36	A16B10	2.16±0.04	3
37	A13B3	2.16±0.12	3
38	A11B12	2.14±0.07	4
39	A7B2	2.14±0.13	4
40	A4B12	2.11±0.05	4
41	A19B3	2.09±0.10	4
42	A12B10	2.07±0.07	4
43	A14B14	2.02±0.12	3
44	A21B2	2.00±0.10	3
45	A13B2	2.00±0.05	4
46	A20B2	1.97±0.05	4
47	A9B8	1.96±0.07	3
48	A16B5	1.96±0.08	4
49	A14B10	1.94±0.05	4
50	A10B3	1.94±0.06	4
51	A8B8	1.94±0.04	4
52	A14B5	1.93±0.05	4
53	A15B19	1.93±0.10	4
54	A4B2	1.93±0.06	4
55	A14B2	1.92±0.12	4
56	A4B20	1.92±0.05	3
57	A6B10	1.87±0.05	4
58	A7B10	1.84±0.09	4
59	A21B10	1.81±0.08	4
60	A21B11	1.75±0.03	4
61	A9B10	1.60±0.02	4

Bold letters represented the strains selected for fruiting body production.

Table 2: Comparison of the production yields and mycelial colonization times of fruiting bodies.

Strain	Weight of fruiting body (g)	Biological efficiency (%)	Mycelial colonization time (days)
HS5 (Parental strain) strain)	134.57±5.45 b ^*	40.78±1.65 b	30.82±0.98 a
HS4 (Parental strain) strain)	91.43±5.24 cd	27.71±1.59 cd	24.72±1.01 g
A10B4	165.91±12.56 a	50.28±3.81 a	25.18±1.33 efg
A14B4	161.87±10.03 a	49.05±3.04 a	26.09±1.70 def
A8B6	123.20±12.84 b	37.33±3.89 b	26.36±1.69 cde
A7B6	102.67±17.46 c	31.11±5.27 c	29.82±1.08 a
A10B5	91.60±10.32 cd	27.76±3.13 cd	24.36±1.03 g
A1B11	74.70±6.59 d	22.64±2.00 d	26.91±0.94 bcd
A1B6	72.57±12.73 de	21.99±3.86 de	27.64±1.12 b
A1B17	54.47±8.43 ef	16.51±2.55 ef	27.27±1.62 cde
A1B12	54.33±14.33 ef	16.46±4.34 ef	27.36±1.43 bc
A1B18	47.33±3.79 f	14.34±1.15 f	25.00±1.18 fg

^* Means in each column followed by the same letters are not significantly different at P < 0.05 according to Duncan’s multiple range tests.

Como citar

copiar

Editora da Universidade Federal de Lavras Editora da UFLA, Caixa Postal 3037 - 37200-900 - Lavras - MG - Brasil, Telefone: 35 3829-1115 - Lavras - MG - Brazil
E-mail: revista.ca.editora@ufla.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Melhoramento de uma linhagem de alto rendimento para cultivo comercial por cruzamento de <i>Pholiota adiposa</i>

PlumX

Mensagem

[1] ^*Corresponding author: wangshouxian2002@163.com