This work was accomplished with eleven PVX isolates: six from national and imported seeds analised at the Virus Indexation Center of Minas Gerais - Brazil (VIC, LAV, UF, NR, SJ, UDI), one from São Paulo state (VEL), two from Rio Grande do Sul state (P21 and P22), one from Santa Catarina state (CAN) and one from the Federal District (BR). The PVX isolates were mechanically inoculated in several hosts plant, in two diferent seasons (September, 2001 and July, 2002), to verify the severity and host symptoms. The diagnosis was done by visual inspection and DAS-ELISA. Attempts to recover the PVX isolates from DAS-ELISA negative plants were also done by inoculation in the indicator plants Gomphrena globosa and Nicotina tabacum TNN. The symptoms shown by the majority of host plants, when inoculated with the eleven isolates, were similar, but Nicotina glutinosa and Physalis floridana showed distinct symptoms when inoculated with P21 and VEL. Probably, the eleven PVX isolates studied in this work have the same origin of the isolates from groups 1 and 3.
Solanum tuberosum; tobacco; strain
