Starting with alkaline protein extract, the Corolase PP was used for preparing six enzymatic rice hydrolysates, with the aim of producing dietary formulations for phenylketonuria treatment. A column containing activated pure charcoal was used for removing phenylalanine (Phe). The Phe contents of rice protein extract and hydrolysates were determined by second derivative spectrophotometry. The final Phe content of the hydrolysates varied from 0.39 to 68.34 mg 100 g-1, corresponding to removal levels from 84% to 100%. The distribution of peptides according to their size was used as an approach for the nutritional evaluation of the hydrolysates. The fractionation of peptides was performed by size-exclusion HPLC, and the rapid correct fraction area method was used for the their quantification. The results indicated that the higher oligopeptide content was obtained when corolase PP was used with an enzyme:substrate ratio of 1% and the initial protein concentration of 1,56 g 100 mL-1.
Enzymatic hydrolysis; phenylalanine removal; dietary supplement
