The regenerative capacity of buds from lateral sprouts of the pineapple cv. Pérola (Ananas comosus (L.) Merr.) was evaluated in liquid and semi-solid medium, under light and dark conditions, with the objective of producing materials for subsequent phase of micropropagation. The explants were established for 60 days in basal MS medium, supplemented with 0.63 μM NAA and 2.5 μM BAP. The statistical design was completely randomized with four treatments (liquid medium with and without filter paper bridge, and semi-solid medium gellified with agar and phytagel®), four repetitions and five explants per experimental unit. Different volumes of liquid medium (2.5 mL, 5.0 mL, and 10.0 mL) were also evaluated in four replications per treatment. More buds were obtained in liquid medium without filter paper bridge, where 80.0% of explants differentiated new shoots. In treatments cultivated in the dark, 68.8% to 87.5% of the explants did not regenerate new shoots. Explants cultivated in volumes of 2.5 mL and 5.0 mL of medium, in the presence of light, were more efficient in the induction and formation of shoots. The cultivation of axillary buds of lateral pineapple sprouts in 2.5 mL or 5.0 mL of MS liquid medium, supplemented with 0.63 μM NAA and 2.5 μM BAP, in the presence of light, represents an adequate protocol for establishing new pineapple shoots for use in subsequent phases of micropropagation.
Pineapple; in vitro culture; regeneration; Ananas comosus
