During the storage of bean grains (Phaseolus vulgaris L.), production may be reduced due to weevils attack such as the species Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera: Bruchidae). This insect attack affects directly the quality of grain and facilitates the entry of pathogens, making them impractical for consumption and trade. In order to find an alternative strategy to control of this pest, we assessed the possible resistance of near isogenic lines containing arcelin, wild lines containing arcelin and cultivars, in laboratory (T= 25±2° C, R.H.= 70±10% and photoperiod= 12h). The assays were conducted in vials containing 10g of bean grains per genotype, releasing seven pairs of the insect and maintaining the infestation during one week. Twenty-one days after the releasing, the grains were evaluated counting the viable eggs. Twenty-five days after the infestation, the evalutions were daily initiated, observing the number of adults emerged, larvae viability, development period (egg to adult) and weight of grains consumed. The assays were performed in a completely randomized design with eight replicates. The genotypes Arc.2, Arc.3, Arc.4, Arc.3S and Ipa 6 showed low levels of oviposition non-preference, being classified as deterrents. The genotypes Arc.1S and Arc.1 expressed high levels of antibiosis; Arc.2, Arc.3 and Arc.4 showed the same mechanism, however in lower levels.
Host plant resistance; stored bean grains; bean weevil; antixenosis; antibiosis
