With the objective to micropropagation the blackberry (Rubus sp.), cv. Ébano, nodal segments of plants in vitro, originated from Embrapa Clima Temperado/CPACT-Pelotas, RS, were inoculated in MS culture medium (0, 50, 100, 150 and 200%), suplemented with different BAP concentrations (0; 0,5; 1,0; 2,0 and 4,0 mg L-1). The pH was adjusted for 5,8 before adding 6 g L-1 agar and before the esterilization at 121ºC and 1 atm for 20 minutes. After the inoculation, the explants were maintained by 60 days, in growth room, 27±1ºC, 35 µM m-2 s-1 and photoperiod 16 hours. The experiment was randomized complete blocks, using three explants by repetition. Better results were obtained with 150% MS medium, where the largest number (7,89) and sprouts (3,99) were observed, added of 1 mg L-1 de BAP. In the absence of BAP the number of roots had presented better development, without the callus's formation.
Rubus sp.; BAP; culture medium MS
