Uso do acetato de celulose diluido em acido acetico, para tomar impressões dos estomas foliares
The use of cellulose acetate dissolved in acetic acid to take stomata impressions
Dixier M. Medina
Summary
Various substances were tried for obtaining stomata impressions from leaves. The best results were obtained with cellulose acetate dissolved in acetic acid.
The solution was prepared by dissolving 1.2 gra (about 20 cm2 of a 0.15 mm thick commercial sheet) of cellulose acetate in 8 ml of glacial acetic acid. The viscous fluid thus obtained was applied to the leaf surface and after drying produced a thin film that showed the stomata impressions. These films can be stored indefinitely and be mounted in water for microscopical examination.
This method has been used with excellent results for counting stomata of Coffca, Triticum, Sccale, Cinchona, Mamhot, Hevea, and other species.
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Recebida para publicação em 6 de fevereiro de 1961
Datas de Publicação
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Publicação nesta coleção
09 Mar 2010 -
Data do Fascículo
1961
