Morfologia e aspectos intracelulares do vírus latente da couve

Kitajima, E. W.; Camargo, I. J. B.; Costa, A. S.

doi:10.1590/S0006-87051970000100017

Resumos

Partículas alongadas de 15 mµ de diâmetro e 650 mµ de comprimento normal foram consistentemente detectadas em exames electrono--microscópicos de preparações "leaf dip" de 6 espécies diferentes de plantas infetadas pelo vírus latente da couve (Brassica oleracea L. var. acephala) (VLC). Tais partículas são consideradas como sendo de um vírus que poderia ser incluído no grupo do vírus S da batata, na classificação dos vírus alongados de Brandes. Exames de secções ultrafinas de tecidos foliar e radicular de couve infetada pelo VLC, demonstraram a ocorrência do VLC, formando agregados mais ou menos organizados no citoplasma. Não se notou alterações citológicas sensíveis como conseqüência da infecção, o que está de acordo com a ausência de sintomas externos em couve infetada pelo VLC.

Electron microscopic examination of leaf dip preparations made from cole (Brassica oleraceaL. var. acephala), Chenopodium amaranticolorCoste & Reyn., radish (Raphanus sativusL.), mustard(Brassica albaBoiss. Rabenh.), bean (Phaseolus vulgaris L.)and Gomphrena globosaL. plants infected with cole latente virus (CLV) demonstrated consistently the presence of slender rods. These particles, considered as CLV, were about 15 mµ wide and 650 mµ in normal length (NL). The NL was determined in a series of comparative measurements using turnip mosaic virus (NL = 750 mµ) as a standard. Morphological characteristics of CLV permit its inclusion in the potato virus S group, in Brandes' system of classification of elongated plant viruses. Thin section profiles of root and leaf tissues, from CLV-infected cole, showed the frequent occurrence of fibrillar aggregates in the cytoplasm. The particles component of these aggregates were identified as CLV in situ due to the constant association with the infection and their mophological similarities with the particles found in leaf dip preparations. CLV-particles appeared with their longer axis laying side--by-side within these aggregates, often forming a spiral arrangement. Vesiculated areas were occasionally seen in the cytoplasm, with virions in or around them. Virus particles were found in most of the examined cells, except within the tracheids and sieve tubes in the leaf, and within the meristematic zone, in th root. Besides these abnormalities, no other remarkable changes in normal cell structure could be noticed, in agreement with the lack of external symptoms.

Morfologia e aspectos intracelulares do vírus latente da couve¹ 1 Êste trabalho recebeu subvenções da FAPESP (C. Agron.69/879 e 70/076), do CNPq (TC 12275) e do convênio CAPES/BID/BNDE.

Particle morphology and intracellular behavior of cole latent virus

E. W. Kitajima^I; I. J. B. Camargo^II; A. S. Costa^III,² 2 Bolsistas CNPq

^IEngenheiro-agrônomo

^IIBiologista

^IIIEngenheiro-agrônomo, Seção de Virologia, Instituto Agronômico

SINOPSE

Partículas alongadas de 15 mµ de diâmetro e 650 mµ de comprimento normal foram consistentemente detectadas em exames electrono--microscópicos de preparações "leaf dip" de 6 espécies diferentes de plantas infetadas pelo vírus latente da couve (Brassica oleracea L. var. acephala) (VLC). Tais partículas são consideradas como sendo de um vírus que poderia ser incluído no grupo do vírus S da batata, na classificação dos vírus alongados de Brandes.

Exames de secções ultrafinas de tecidos foliar e radicular de couve infetada pelo VLC, demonstraram a ocorrência do VLC, formando agregados mais ou menos organizados no citoplasma. Não se notou alterações citológicas sensíveis como conseqüência da infecção, o que está de acordo com a ausência de sintomas externos em couve infetada pelo VLC.

SUMMARY

Electron microscopic examination of leaf dip preparations made from cole (Brassica oleraceaL. var. acephala), Chenopodium amaranticolorCoste & Reyn., radish (Raphanus sativusL.), mustard(Brassica albaBoiss. Rabenh.), bean (Phaseolus vulgaris L.)and Gomphrena globosaL. plants infected with cole latente virus (CLV) demonstrated consistently the presence of slender rods. These particles, considered as CLV, were about 15 mµ wide and 650 mµ in normal length (NL). The NL was determined in a series of comparative measurements using turnip mosaic virus (NL = 750 mµ) as a standard. Morphological characteristics of CLV permit its inclusion in the potato virus S group, in Brandes' system of classification of elongated plant viruses.

Thin section profiles of root and leaf tissues, from CLV-infected cole, showed the frequent occurrence of fibrillar aggregates in the cytoplasm. The particles component of these aggregates were identified as CLV in situ due to the constant association with the infection and their mophological similarities with the particles found in leaf dip preparations. CLV-particles appeared with their longer axis laying side--by-side within these aggregates, often forming a spiral arrangement. Vesiculated areas were occasionally seen in the cytoplasm, with virions in or around them. Virus particles were found in most of the examined cells, except within the tracheids and sieve tubes in the leaf, and within the meristematic zone, in th root. Besides these abnormalities, no other remarkable changes in normal cell structure could be noticed, in agreement with the lack of external symptoms.

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LITERATURA CITADA

Recebido para publicação em 25 de março de 1970

1. BODE, O. & BRANDES, J. Elektronenmikroskopische Untersuchungen des Kohlrübenmosaik-Virus (Turnip mosaic virus). Phytopath. Z. 34:103-106, 1958.
2. BORGES, M. L. V. & DAVID-FERREIRA, J. F. Comparative study of cell structure in Datura metel L. healthy and infected with potato virus X or potato virus Y. Revta. Biol. 6:421-437, 1968.
3. BRANDES, J. Eine elektronenmikroskopische Schnellmethode zum Nachweis faden-und stabchenformiger Viren, insbesondere in Kartoffeldunkelkeimen. NachrBl. dt. PflSchutzdienst, Braunschweig, 9:151-152, 1957.
⁴
________. Identifizierung von gestreckten Pflanzen pathogen Viren auf morfologischer Grundlage. Mitt. biol. Bundesanstalt, Berlin-Dahlem, 110. 1964. 130p.
5. DE ZOETEN, G. A. & SCHLEGEL, D. E. Nucleolar and cytoplasmic uridine-H³ incorporation in virus infected plants. Virology 32:416-427, 1967.
6. EDWARDSON, J. R. Cylindrical inclusions in the cytoplasm of leaf cells infected with tobacco etch virus. Science 153:883-884, 1964.
7. KIKUMOTO, T. & MATSUI, C. Electron microscopy of intra-cellular potato virus X. I. Virology 13:294-299, 1961.
8. KITAJTMA, E. W. A rapid method to detect particles of some spherical plant viruses in fresh preparations. J. Electronmi-croscopy, Tokyo, 14:119-121, 1965.
9. KITAJIMA, E. W. Electron microscopy of vira-cabeça virus (Brazilian tomato spotted wilt virus) within host cell. Virology 26:89-99, 1965.
10. ________ & COSTA, A. S. Microscopia electrônica de tecidos foliares de mandioca infetados pelo vírus do mosaico comum da mandioca. Bragantia 25:XXIII-XXVIII, 1966. Nota 6.
11. KOHLEMAINEN, L.; ZECH, H. & WETTSTEIN, D. VON. The structure of cells during tobacco mosaic reproduction. Me-sophyll cells containing virus crystals. J. Cell Biol. 25 (part 2): 77-97, 1965.
12. LUFT, J. H. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9:409-414, 1961.
13. LYONS, A. R. & ALLEN JR., T. C. Electron microscopy of virus-like particles associated with necrotic fleck of Lilium longi-florum. J. Ultrastruct. Res. 27:198-204, 1969.
14. MILLONING, G. Studio sui fattori che determinano la preservazione della ultrastruttura. In: Buffa, P., ed. From molecule to cell. Symposia on Electron Microscopy. Roma, C. N. R. 1964. p. 347-362.
15. PURCIFULL, D. E.; EDWARDSON, J. R. & CHTISTIE, R. G. Electron microscopy of intracellular aggregates in pea (Pisum sativum) infected with clover yellow mosaic virus. Virology 29:276-284, 1966.
16. REYNOLDS, E. S. The use of lead citrate at higt pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:208-211, 1963.
17. SCHNEPF, E. & BRANDES, J. Uber ein Virus aus Passiflora spec Phytopath. Z. 43:102-105, 1961/62.
18. SHALLA, T. A. Assembly and aggregation of tobacco mosaic virus in tomato leaflets. J. Cell Biol. 21:253-264, 1964.
19. WATSON, M. L. Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4:475-478, 1958.

1

Êste trabalho recebeu subvenções da FAPESP (C. Agron.69/879 e 70/076), do CNPq (TC 12275) e do convênio CAPES/BID/BNDE.

2

Bolsistas CNPq

Datas de Publicação

Publicação nesta coleção
18 Mar 2009
Data do Fascículo
Jan 1970

Histórico

Recebido
25 Mar 1970

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BODE, O. & BRANDES, J. Elektronenmikroskopische Untersuchungen des Kohlrübenmosaik-Virus (Turnip mosaic virus). Phytopath. Z. 34:103-106, 1958.

[2] 2. BORGES, M. L. V. & DAVID-FERREIRA, J. F. Comparative study of cell structure in Datura metel L. healthy and infected with potato virus X or potato virus Y. Revta. Biol. 6:421-437, 1968.

[3] 3. BRANDES, J. Eine elektronenmikroskopische Schnellmethode zum Nachweis faden-und stabchenformiger Viren, insbesondere in Kartoffeldunkelkeimen. NachrBl. dt. PflSchutzdienst, Braunschweig, 9:151-152, 1957.

[4] ⁴
________. Identifizierung von gestreckten Pflanzen pathogen Viren auf morfologischer Grundlage. Mitt. biol. Bundesanstalt, Berlin-Dahlem, 110. 1964. 130p.

[5] 5. DE ZOETEN, G. A. & SCHLEGEL, D. E. Nucleolar and cytoplasmic uridine-H³ incorporation in virus infected plants. Virology 32:416-427, 1967.

[6] 6. EDWARDSON, J. R. Cylindrical inclusions in the cytoplasm of leaf cells infected with tobacco etch virus. Science 153:883-884, 1964.

[7] 7. KIKUMOTO, T. & MATSUI, C. Electron microscopy of intra-cellular potato virus X. I. Virology 13:294-299, 1961.

[8] 8. KITAJTMA, E. W. A rapid method to detect particles of some spherical plant viruses in fresh preparations. J. Electronmi-croscopy, Tokyo, 14:119-121, 1965.

[9] 9. KITAJIMA, E. W. Electron microscopy of vira-cabeça virus (Brazilian tomato spotted wilt virus) within host cell. Virology 26:89-99, 1965.

[10] 10. ________ & COSTA, A. S. Microscopia electrônica de tecidos foliares de mandioca infetados pelo vírus do mosaico comum da mandioca. Bragantia 25:XXIII-XXVIII, 1966. Nota 6.

[11] 11. KOHLEMAINEN, L.; ZECH, H. & WETTSTEIN, D. VON. The structure of cells during tobacco mosaic reproduction. Me-sophyll cells containing virus crystals. J. Cell Biol. 25 (part 2): 77-97, 1965.

[12] 12. LUFT, J. H. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9:409-414, 1961.

[13] 13. LYONS, A. R. & ALLEN JR., T. C. Electron microscopy of virus-like particles associated with necrotic fleck of Lilium longi-florum. J. Ultrastruct. Res. 27:198-204, 1969.

[14] 14. MILLONING, G. Studio sui fattori che determinano la preservazione della ultrastruttura. In: Buffa, P., ed. From molecule to cell. Symposia on Electron Microscopy. Roma, C. N. R. 1964. p. 347-362.

[15] 15. PURCIFULL, D. E.; EDWARDSON, J. R. & CHTISTIE, R. G. Electron microscopy of intracellular aggregates in pea (Pisum sativum) infected with clover yellow mosaic virus. Virology 29:276-284, 1966.

[16] 16. REYNOLDS, E. S. The use of lead citrate at higt pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:208-211, 1963.

[17] 17. SCHNEPF, E. & BRANDES, J. Uber ein Virus aus Passiflora spec Phytopath. Z. 43:102-105, 1961/62.

[18] 18. SHALLA, T. A. Assembly and aggregation of tobacco mosaic virus in tomato leaflets. J. Cell Biol. 21:253-264, 1964.

[19] 19. WATSON, M. L. Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4:475-478, 1958.

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Morfologia e aspectos intracelulares do vírus latente da couve

Particle morphology and intracellular behavior of cole latent virus

Resumos

Datas de Publicação

Histórico