Effects of harvesting practices and gin machinery on the properties of cotton fibers and yarn were studied for three commercial varieties: IAC 13-1, IAC RM3, and IAC RM4 SM5. There was no difference in the majority of technological properties, between hand-picked cotton and machine-picked cotton. However, machine-picked cotton had better fiber length in Paranapanema-SP and better Micronaire in Araras-SP. Roller gin was better than saw gin for all technological cotton fiber properties and yarn, independent of harvesting processes.