This paper deals with the occurrence of natural enemies of Mocis latipes (Guenée) (Lepidoptera: Noctuidae) in Campinas and Piracicaba, State of São Paulo, Brazil. Larvae and pupae were collected in the field and kept in laboratory until the emergence of the parasites. Patelloa similis (Townsend) and Euphorocera floridensis Townsend (Diptera: Tachinidae) were observed attacking M. latipes and the former being predominant at Campinas. A hyperparasite Brachymeria ovata (Say) emerged from the pupae of these tachinid flies. At the end of M. latipes infestation in the field a predator, Polistes canadensis (Hymenoptera: Vespidae), was observed attacking the caterpillars. At Piracicaba, the parasites noted were ichneumon Netelia sp. and tachinid P. similis. At this location Netelia sp. is being observed attacking M. latipes for the first time in Brazil.