Aiming to identify parents and hybrids among Vitis vinifera (bunch grapes) and Vitis rotundifolia (muscadine grapes) isozyme polymorphism and RAPD marker were used. GOT (glutamate oxaloacetate transaminase), IDH (isocitrate dehydrogenase) and PGI (phosphoglucoisomerase) systems could differentiate the muscadine, being identified five allozymes for GOT, two allozymes for LAP (leucine aminopeptidase) and four allozymes for EST (esterase). PGI and IDH systems were dimeric and had the phenotype of four allozymes in two regions and three allozymes in one region, respectivelly. RAPD marker showed polymorphism that led to the differentiation among all genotypes. UPGMA dendrogram obtained through isozymes and RAPD marker were similar. The strongest linkage was found among 'Italia' and 'Rubi', followed by 'Patricia' and 'A Dona'. Piratininga and Eugênio cultivars were also closely linked to the others. Through isozymes and RAPD marker, muscadine was found very distantely and separately related to the other groups. When RAPD was used for muscadines, the NC66C203-9 hybrid, one possible hybrid and its female parent, the hybrids were located in an intermediate position for muscadine and bunch grapes, however the possible hybrid was similar to its female parent, while the NC66C203-9 presented bands from both muscadine and bunch grapes, confirming its hybrid origin.
grapevine; breeding enzymatic polimorphism; RAPD; interspecific crossing; disease resistance