Chemical composition, phytotoxic, and cytogenotoxic properties of essential oils from <i>Psidium cauliflorum</i> and <i>P. acidum</i> (Myrtaceae)

Alves, Thammyres de Assis; Venancio, Aldino Neto; Alves, Thayllon de Assis; Vasconcelos, Loren Cristina; Tuler, Amélia Carlos; Silva, Matheus Alves; Radael, Lara Aparecida Nazareth; Menini, Luciano; Ferreira, Marcia Flores da Silva; Praça-Fontes, Milene Miranda

doi:10.1590/1678-4499.20230180

Thammyres de Assis Alves

Conceptualization

Methodology

Investigation

Writing – original draft

Universidade Federal do Espírito Santo – Centro de Ciências Exatas, Naturais e da Saúde – Departamento de Biologia – Alegre (ES), Brazil.

http://orcid.org/0000-0001-7697-4184

Aldino Neto Venancio

Methodology

Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo – Laboratório de Química Aplicada – Alegre (ES), Brazil.

http://orcid.org/0000-0002-7217-1066

Thayllon de Assis Alves

Methodology

Writing – original draft

Universidade Federal do Espírito Santo – Centro de Ciências Exatas, Naturais e da Saúde – Departamento de Biologia – Alegre (ES), Brazil.

http://orcid.org/0000-0003-2478-851X

Loren Cristina Vasconcelos

Methodology

Investigation

Writing – review & editing

Universidade Federal do Espírito Santo – Centro de Ciências Exatas, Naturais e da Saúde – Departamento de Biologia – Alegre (ES), Brazil.

http://orcid.org/0000-0002-0268-8529

Amélia Carlos Tuler

Methodology

Investigation

Writing – review & editing

Instituto Nacional da Mata Atlântica – Santa Teresa (ES), Brazil.

http://orcid.org/0000-0002-1512-5493

Matheus Alves Silva

Methodology

Investigation

Universidade Federal do Espírito Santo – Centro de Ciências Agrárias e Engenharias - Departamento de Agronomia – Alegre (ES), Brazil.

http://orcid.org/0000-0002-4992-9588

Lara Aparecida Nazareth Radael

Methodology

Universidade Federal do Espírito Santo – Centro de Ciências Exatas, Naturais e da Saúde – Departamento de Biologia – Alegre (ES), Brazil.

http://orcid.org/0009-0003-8273-7347

Luciano Menini

Investigation

Writing – review & editing

Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo – Laboratório de Química Aplicada – Alegre (ES), Brazil.

http://orcid.org/0000-0003-3656-5428

Marcia Flores da Silva Ferreira

Investigation

Writing – review & editing

Supervision

Universidade Federal do Espírito Santo – Centro de Ciências Agrárias e Engenharias - Departamento de Agronomia – Alegre (ES), Brazil.

http://orcid.org/0000-0003-1541-6634

Milene Miranda Praça-Fontes ^**Corresponding author: milenemiranda@yahoo.com.br

Conceptualization

Investigation

Writing – review & editing

Supervision

Universidade Federal do Espírito Santo – Centro de Ciências Exatas, Naturais e da Saúde – Departamento de Biologia – Alegre (ES), Brazil.

http://orcid.org/0000-0001-7738-9518

*Corresponding author: milenemiranda@yahoo.com.br

Section Editor: Gabriel Constantino Blain https://orcid.org/0000-0001-8832-7734

CONFLICT OF INTEREST

Nothing to declare.

Figures (5)
Tables (2)

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Figure 1
Effect of the essential oil of Psidium acidum and Psidium cauliflorum on the (a) germination percentage and on the (b) germination speed index of Lactuca sativa and Sorghum bicolor. The different lowercase letters above the boxplots indicate significant difference between the treatments by Tukey’s test (p < 0.05).

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Figure 2
Effect of the essential oil of Psidium acidum and Psidium cauliflorum on the (a) root growth and on the (b) shoot length of Lactuca sativa and Sorghum bicolor. The different lowercase letters above the boxplots indicate significant difference between the treatments by Tukey’s test (p < 0.05).

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Figure 3
Mitotic index for Lactuca sativa treated with solutions of Psidium acidum and Psidium cauliflorum essential oils.

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Figure 4
Nuclear and chromosomal alterations were observed in the root meristems of lettuce treated with the essential oils of Psidium acidum and Psidium cauliflorum at concentrations of 3,000, 1,500, 750, 375, and 187.5 mg·mL^-1. The different lowercase letters above the boxplots indicate significant differences between the treatments, as determined by Tukey’s test (p < 0.05).

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Figure 5
Frequency of each chromosomal alterations observed in meristematic cells of lettuce treated with essential oils of Psidium acidum and of the Psidium cauliflorum at the concentrations of 3,000, 1,500, 750, 375 and 187.5 mg·mL^-1. The different lowercase letters above the boxplots indicate significant difference between the treatments by Tukey’s test (p < 0.05).

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Table 1
Identification of the compounds present in Psidium cauliflorum essential oil using the linear temperature programmed retention indexes and gas chromatography-mass spectrometry^a a The compounds were identified by the LTPRI index (GC / FID) and by mass spectrometry using a column Rtx®-5MS; .

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Table 2
Identification of the compounds present in Psidium acidum essential oil using the linear temperature programmed retention indexes and gas chromatography-mass spectrometry^a a The compounds were identified by the LTPRI index (GC / FID) and by mass spectrometry using a column Rtx®-5MS; .

Compound^b b tabulated retention index (Adams, 2007; NIST, 2011; El-Sayed, 2016);	Retention time (min)	Relative area (%)^c c only compounds with relative areas > 2% were identified;	Calculated retention index^d d retention index calculated from data obtained by sampling saturated n-alkanes (C7-C40).	Literature retention index
α-Pinene	8.369	49.16	932	932
D-Limonene	12.421	4.82	1,027	1,024
Î²-Ocimene	13.434	3.19	1,049	1,044
Borneol	18.765	4.24	1,165	1,165
α-Terpineol	19.966	7.45	1,191	1,186
α-Copaene	28.107	11.39	1,374	1,374
α-Bisabolene	33.644	3.05	1,508	1,506
Î”-Cadinene	34.205	3.65	1,523	1,522
(E)-Nerolidol	35.841	13.05	1,565	1,561

Compound^b b tabulated retention index (Adams, 2007; NIST, 2011; El-Sayed, 2016);	Retention time (min)	Relative area (%)^c c only compounds with relative areas > 2% were identified;	Calculated retention index^d d retention index calculated from data obtained by sampling saturated n-alkanes (C7-C40);	Literature retention index
α-Pinene	8.344	4.19	931	932
Î²-Pinene	10.085	2.17	973	974
Î²-cis-Ocimene	13.438	2.06	1,049	1,044
α-Copaene	28.124	11.67	1,374	1,374
Î²-Elemene	28.871	18.36	1,391	1,389
trans-Caryophyllene	29.943	18.43	1,417	1,417
α-Humulene	31.305	2.56	1,451	1,452
Î³-Muurolene	32.456	2.83	1,479	1,478
Î²-Selinene	32.649	2.82	1,483	1,489
Î”-selinene	33.036	4.22	1,493	1,492
germacrene A	33.467	16.83	1,504	1,508
α-Farnesene	33.679	2.00	1,509	1,505
Î”-Cadinene	34.215	3.66	1,523	1,522
Caryophyllene oxide	36.420	2.52	1,580	1,582
NI^e e unidentified compound.	39.175	5.68	1,654	-

[1] *Corresponding author: milenemiranda@yahoo.com.br

Brasil

Brasil

Chemical composition, phytotoxic, and cytogenotoxic properties of essential oils from Psidium cauliflorum and P. acidum (Myrtaceae)

ABSTRACT