Data are presented on the variation of the level of dry matter, cyanide, starch, total soluble carbohydrates, and reducing sugars content in roots of 13 months old cassava (Manihot esculenta, Crantz) at successive stages after the total removal of the aereal portions. During the 14 days following pruning starch percentage decreased from 79.06% to 62.52%; total soluble carbohydrates and reducing sugars showed a variation from 7.65% and 1.55% up to 17.56% and 5.03% respectively. Total carbohydrate presented small losses by pruning probably due to the new sprouting process. Root cyanid content decreased from 67 to 35 ppm. Its has also been observed that at the 17th day after pruning, different levels of similar carbohydrates were recorded in the different root types as those from the base and the middle of the planted cutting and those from the plant stem base. Such variations are hypothetically ascribed to either a more intensive mobilization of stored carbohydrates or to larger differences of age and composition of each type of root since its origin. This suggests more detailed research work about root sampling method for laboratory analysis.