The influence of the repeated applications of fertilizer mixtures containing P and S and of mixtures without S, was studied by a permanent field trial conducted with 'IAC 16' cotton, during four years, on Latosolic B "Terra Roxa", acid and of low fertility. Through the combination of various fertilizers, as ammonium sulfate, ammonium nitrate, sodium nitrate (Chilean product), ordinary superphosphate, concentrated superphosphate and potassium chloride, N and K were supplied in constant doses, and P and S in variable doses. Sulfur was used always in the presence of phosphorous. Lime was applied in a small dose, before the first and the last years' planting. The experimental design was a Latin square, 6x6. The risk of unsuccess in cotton culture conducted immediately after liming of acid soils, was confirmed by the low levels of productivity observed in the first year, even at higher dosages of fertilizers. In this year, the effect of P on cotton yield was not significant and it was quadratic in the successive years. Sulfur effect was appreciable already in the first year, and it increased with time. Ordinary superphosphate behaved as a mixed fertilizer, since it increased the calcium level of soil analysis and the level of Ca and S of the leaf blade of cotton. No correlation between cotton yield and leaf blade nutrient concentration was observed, except for sulfur.