An investigation of the presence of specific anaerobic species in necrotic primary teeth

Gomes, Genara Brum; Sarkis-Onofre, Rafael; Bonow, Maria Laura Menezes; Etges, Adriana; Jacinto, Rogério Castilho

doi:10.1590/S1806-83242013000100020

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Pediatric Dentistry • Braz. Oral Res. 27 (2) • Mar-Apr 2013 • https://doi.org/10.1590/S1806-83242013000100020 copy

An investigation of the presence of specific anaerobic species in necrotic primary teeth

Authorship SCIMAGO INSTITUTIONS RANKINGS

Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The “red complex”, which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.

Tooth, Deciduous; Endodontics; Bacteria, Anaerobic; Polymerase Chain Reaction

Species	Sequence	Size (bp)	A.T.
Non-specific (EuF/EuR)	5′ TCC TAC GGG AGG CAG CAG T 3′ 5′ GGA CTA CCA GGG TAT CTA ATC CTG TT 3′	466	60°C
F. alocis	5′ AAA CCC ATC TCT GAG TTC TTC TTC 3′ 5′ ATG CCA ACT TGA CGT TAA AT 3′	594	60°C
F. nucleatum	5′ ATT GTG GCT AAA AAT TAT AGT T 3′ 5′ ACC CTC ACT TTG AGG ATT ATA G 3′	1000	55°C
P. micra	5′ AGA GTT TGA ATC CTG GCT CAG 3′ 5′ ATA TCA TGC GAT TCT GTG GTC TC 3′	207	60°C
P. endodontalis	5′ GCT GCA GCT CAA CTG TAG TC 3′ 5′ CCG CTT CAT GTC ACC ATG TC 3′	672	58°C
P. gingivalis	5′ AGG CAG CTT GCC TAG AGT CGG 3′ 5′ ACT GTT AGC AAC TAC CGA TGT 3′	404	58°C
P. intermedia	5′ TTT GTT GGG GAG TAA AGC GGG 3′ 5′ TCA ACA TCT CTG TAT CCT GCG T 3′	575	58°C
P. nigrescens	5′ ATG AAA CAA AGG TTT TCC GGT AAG 3′ 5′ CCC ACG TCT CTG TGG GCT GCG A 3′	804	58°C
P. tannerae	5′ CTT AGC TTG CTA AGT ATG CCG 3′ 5′ CAG CTG ACT TAT ACT CCC G 3′	550	55°C
T. forsythia	5′ TGC TTC AGT AGT TAT ACC T 3′ 5′ TGC TTC AGT GTC AGT TAT ACC T 3′	641	56°C
T. denticola	5′ TAA TAC CGA ATG TGC TCA TTT ACA T 3′ 5′ TCA AAG AAG CAT TCC CTC TTC TTC TTA 3′	316	60°C
T. socranskii	5′ GAT CAC TGT ATA CGG AAG GTA GAC A 3′ 5′ TAC ACT TAT TCC TCG GAC AG 3′	288	56°C

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[1] Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.