Immunohistochemical study of the plasminogen activator system in benign epithelial odontogenic lesions

Costa, Carla Samily de Oliveira; Mafra, Rodrigo Porpino; Rolim, Larissa Santos Amaral; Souza, Lélia Batista de; Pinto, Leão Pereira

doi:10.1590/1807-3107bor-2022.vol36.0071

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original Research, Oral Pathology • Braz. oral. res. 36 • 2022 • https://doi.org/10.1590/1807-3107bor-2022.vol36.0071 copy

Immunohistochemical study of the plasminogen activator system in benign epithelial odontogenic lesions

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

The aim of this study was to analyze and compare the immunohistochemical expression of plasminogen activator system (PAS) proteins (uPA, uPAR, and PAI-1) in ameloblastomas (AMBs), odontogenic keratocysts (OKCs), and dental follicles (DFs) representing normal odontogenic tissue, as well as to investigate possible correlations between these proteins. Twenty AMBs, 20 OKCs, and 10 DFs were selected for immunohistochemical analysis. In each case, the immunoexpression of uPA, uPAR, and PAI-1 was evaluated semiquantitatively based on the percentage of positivity in odontogenic epithelial and connective tissue cells. The epithelial immunoexpression of uPA was significantly lower in AMBs when compared to OKCs (p = 0.001) and DFs (p = 0.029). Significantly higher epithelial immunostaining for uPAR was observed in AMBs when compared to OKCs (p < 0.001). There were no significant differences in the epithelial immunoexpression of PAI-1 between AMBs and OKCs (p = 1.000). The correlations found for the expression of the studied proteins were not statistically significant (p > 0.05). However, the epithelial and connective tissue expressions of uPAR have a strong positive and statistically significant correlation in AMBs. The present results suggest that uPA is involved in the pathogenesis of OKCs and that uPAR may participate in tumorigenesis in AMBs. The high percentage of PAI-1-positive cells suggests a possible role for this protein in the development of AMBs and OKCs. Furthermore, the studied proteins do not seem to act synergistically in AMBs, OKCs, and DFs.

Keywords:
Urokinase-type Plasminogen Activator; Plasminogen Inactivators; Odontogenic Tumors; Odontogenic Cysts; Immunohistochemistry

Biomarker/ score		Group
		Ameloblastoma	Odontogenic keratocyst	Dental follicle
		n (%)	n (%)	n (%)
uPA
Immunopositivity
	0 (0%)	1 (5.0)	0 (0.0)	1 (10.0)
	1 (1–10%)	0 (0.0)	0 (0.0)	0 (0.0)
	2 (11–50%)	5 (25.0)	1 (5.0)	0 (0.0)
	3 (51–80%)	6 (30.0)	1 (5.0)	0 (0.0)
	4 (> 80%)	8 (40.0)	18 (90.0)	9 (90.0)
Intensity
	Absent	1 (5.0)	0 (0.0)	1 (10.0)
	Weak	15 (75.0)	9 (45.0)	5 (50.0)
	Moderate	4 (20.0)	11 (55.0)	4 (40.0)
	Strong	0 (0.0)	0 (0.0)	0 (0.0)
uPAR
Immunopositivity
	0 (0%)	2 (10.0)	15 (75.0)	6 (60.0)
	1 (1–10%)	14 (70.0)	5 (25.0)	0 (0.0)
	2 (11–50%)	2 (10.0)	0 (0.0)	1 (10.0)
	3 (51–80%)	0 (0.0)	0 (0.0)	2 (20.0)
	4 (> 80%)	2 (10.0)	0 (0.0)	1 (10.0)
Intensity
	Absent	2 (10.0)	15 (75.0)	6 (60.0)
	Weak	14 (70.0)	3 (15.0)	4 (40.0)
	Moderate	4 (20.0)	1 (5.0)	0 (0.0)
	Strong	0 (0.0)	1 (5.0)	0 (0.0)
PAI-1
Immunopositivity
	0 (0%)	0 (0.0)	0 (0.0)	0 (0.0)
	1 (1–10%)	0 (0.0)	0 (0.0)	0 (0.0)
	2 (11–50%)	0 (0.0)	0 (0.0)	0 (0.0)
	3 (51–80%)	1 (5.0)	1 (5.0)	0 (0.0)
	4 (> 80%)	19 (95.0)	19 (95.0)	10 (100.0)
Intensity
	Absent	0 (0.0)	0 (0.0)	0 (0.0)
	Weak	4 (20.0)	5 (25.0)	1 (10.0)
	Moderate	14 (70.0)	10 (50.0)	6 (60.0)
	Strong	2 (10.0)	5 (25.0)	3 (30.0)
	Total	20 (100.0)	20 (100.0)	10 (100.0)

Biomarker/ group		n	Median	Q₂₅–Q₇₅	Mean of ranks	KW	p-value
uPA
	Ameloblastoma	20	3.00	2.00–4.00	18.25	12.657	0.002^* * Statistically significant results.
	Odontogenic keratocyst	20	4.00	4.00–4.00	30.58
	Dental follicle	10	4.00	4.00–4.00	29.85
uPAR
	Ameloblastoma	20	1.00	1.00–1.00	33.60	14.847	0.001^* * Statistically significant results.
	Odontogenic keratocyst	20	0.00	0.00–0.75	17.25
	Dental follicle	10	0.00	0.00–3.00	25.80

Biomarker/ group		n	Median	Q₂₅–Q₇₅	Mean of ranks	U	p-value
uPA
	Ameloblastoma	20	3.00	2.00–4.00	15.48	99.50	0.001^* * Statistically significant results.
	Odontogenic keratocyst	20	4.00	4.00–4.00	25.53
	Ameloblastoma	20	3.00	2.00–4.00	13.28	55.50	0.029^* * Statistically significant results.
	Dental follicle	10	4.00	4.00–4.00	19.95
	Odontogenic keratocyst	20	4.00	4.00–4.00	15.55	99.00	0.933
	Dental follicle	10	4.00	4.00–4.00	15.40
uPAR
	Ameloblastoma	20	1.00	1.00–1.00	27.50	60.00	< 0.001^* * Statistically significant results.
	Odontogenic keratocyst	20	0.00	0.00–0.75	13.50
	Ameloblastoma	20	1.00	1.00–1.00	16.60	78.00	0.302
	Dental follicle	10	0.00	0.00–3.00	13.30
	Odontogenic keratocyst	20	0.00	0.00–0.75	14.25	75.00	0.173
	Dental follicle	10	0.00	0.00–3.00	18.00

Biomarker/ score		Group
		Ameloblastoma	Odontogenic keratocyst	Dental follicle
		n (%)	n (%)	n (%)
uPA
Immunopositivity
	0 (0%)	1 (5.0)	0 (0.0)	0 (0.0)
	1 (1–10%)	2 (10.0)	1 (5.0)	2 (20.0)
	2 (11–50%)	10 (50.0)	10 (50.0)	6 (60.0)
	3 (51–80%)	4 (20.0)	7 (35.0)	2 (20.0)
	4 (> 80%)	3 (15.0)	2 (10.0)	0 (0.0)
Intensity
	Absent	1 (5.0)	0 (0.0)	0 (0.0)
	Weak	13 (65.0)	7 (35.0)	7 (70.0)
	Moderate	6 (30.0)	13 (65.0)	3 (30.0)
	Strong	0 (0.0)	0 (0.0)	0 (0.0)
uPAR
Immunopositivity
	0 (0%)	0 (0.0)	0 (0.0)	2 (20.0)
	1 (1–10%)	8 (40.0)	13 (65.0)	5 (50.0)
	2 (11–50%)	9 (45.0)	7 (35.0)	3 (30.0)
	3 (51–80%)	1 (5.0)	0 (0.0)	0 (0.0)
	4 (> 80%)	2 (20.0)	0 (0.0)	0 (0.0)
Intensity
	Absent	0 (0.0)	0 (0.0)	2 (20.0)
	Weak	15 (75.0)	18 (90.0)	8 (80.0)
	Moderate	5 (25.0)	2 (10.0)	0 (0.0)
	Strong	0 (0.0)	0 (0.0)	0 (0.0)
PAI-1
Immunopositivity
	0 (0%)	0 (0.0)	0 (0.0)	0 (0.0)
	1 (1–10%)	1 (5.0)	0 (0.0)	0 (0.0)
	2 (11–50%)	4 (20.0)	2 (10.0)	5 (50.0)
	3 (51–80%)	6 (30.0)	9 (45.0)	2 (20.0)
	4 (> 80%)	9 (45.0)	9 (45.0)	3 (30.0)
Intensity
	Absent	0 (0.0)	0 (0.0)	0 (0.0)
	Weak	8 (40.0)	0 (0.0)	1 (10.0)
	Moderate	11 (55.0)	13 (65.0)	7 (70.0)
	Strong	1 (5.0)	7 (35.0)	2 (20.0)
Total		20 (100.0)	20 (100.0)	10 (100.0)

Biomarker/ group		n	Median	Q₂₅–Q₇₅	Mean of ranks	KW	p-value
uPA
	Ameloblastoma	20	2.00	2.00–3.00	25.20	2.467	0.291
	Odontogenic keratocyst	20	2.00	2.00–3.00	28.40
	Dental follicle	10	2.00	1.75–2.25	20.30
uPAR
	Ameloblastoma	20	2.00	1.00–2.00	30.65	5.771	0.056
	Odontogenic keratocyst	20	1.00	1.00–2.00	23.38
	Dental follicle	10	1.00	0.75–2.00	19.45
PAI-1
	Ameloblastoma	20	3.00	2.25–4.00	25.75	2.567	0.277
	Odontogenic keratocyst	20	3.00	3.00–4.00	28.15
	Dental follicle	10	2.50	2.00–4.00	19.70

Correlation	Ameloblastoma		Odontogenic keratocyst		Dental follicle
Correlation	r	p-value	r	p-value	r	p-value
uPA (Ep) x uPA (CT)	0.357	0.123	0.253	0.282	0.527	0.117
uPAR (Ep) x uPAR (CT)	0.582	0.007^* * Statistically significant results. - It was not possible to perform a statistical test; Ep: epithelial immunopositivity score; CT: connective tissue immunopositivity score.	-0.182	0.444	0.357	0.311
PAI-1 (Ep) x PAI-1 (CT)	0.404	0.077	-0.242	0.304	–	–

Sociedade Brasileira de Pesquisa Odontológica - SBPqO Av. Prof. Lineu Prestes, 2227, 05508-000 São Paulo SP - Brazil, Tel. (55 11) 3044-2393/(55 11) 9-7557-1244 - São Paulo - SP - Brazil
E-mail: office.bor@ingroup.srv.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Corresponding Author: Carla Samily de Oliveira Costa E-mail: carlasamily@hotmail.com