Association of MTR and MTRR genes and oral health-related quality of life in children with dental caries

ANTUNES, Lívia Azeredo Alves; FIALHO, Walter; FERNANDES, Thais de Oliveira; DUARTE, Camila; TAVARES, Jhenyfer da Silva; KÜCHLER, Erika Calvano; ANTUNES, Leonardo Santos

doi:10.1590/1807-3107bor-2023.vol37.0076

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Original Research, Behavioral Sciences • Braz. oral. res. 37 • 2023 • https://doi.org/10.1590/1807-3107bor-2023.vol37.0076 copy

Association of MTR and MTRR genes and oral health-related quality of life in children with dental caries

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

This study aimed to assess whether genetic polymorphisms in MTR and MTRR are potential biomarkers of oral health-related quality of life (OHRQoL) in children with caries. A cross-sectional study was designed wherein pairs of parents/caregivers and children (aged two–five years) were selected. Clinical examination was used to detect dental caries, which were classified as low-severity and high-severity caries. The Early Childhood Oral Health Impact Scale (ECOHIS) questionnaire was used to assess OHRQoL. Genomic DNA extracted from the saliva was used to analyze two missense genetic polymorphisms: MTR (rs1805087) and MTRR (rs1801394). Mann–Whitney non-parametric test was used to analyze candidate genes with OHRQoL scale and domain, with a significance level of p≤0.05. MTR (rs1805087) was found associated (p = 0.05) with children’s OHRQoL subscale scores in the dominant model (GG + AG). Genetic polymorphisms in MTR may increase the risk of poor OHRQoL in children with caries. Further studies are needed to investigate genetics, molecular factors, and OHRQoL.

Genetics; Dental caries; Oral health; Quality of life, children

Gene (polymorphism)	Position	Functional consequence	Ref SNP alleles	MAF
MTR (rs1805087)	Chr.1: 236885200 (GRCh38)	Missense variant, coding sequence variant: Asp919Gly	A/G	G = 0.2182
MTRR (rs1801394)	Chr.5: 7870860 (GRCh38)	Missense variant, coding sequence variant: Met22Ile	A/G	G = 0.3642

Variables	Caries experience groups

Oral Health-related Quality of life (OHRQoL)	Low caries experience (n = 92)		High caries experience (n = 38)		p-value

	Median	Mean	Median	Mean

	(Q1-Q3)	(SD)	(Q1-Q3)	(SD)
Child Subscale	3.00 (1.00-6-00)	3.90 (3.79)	4.00 (2.00-7.00)	4.55 (3.15)	0.12
D1 – Symptoms	0.00 (0.00-0.00)	0.39 (0.79)	0.00 (0.00-1.00)	0.55 (0.89)	0.28
D2 – Function	2.00 (0.00-3.00)	1.89 (1.92)	2.50 (1.00-4.00)	2.86 (2.20)	0.01
D3 – Psychological	0.00 (0.00-2.00)	1.23 (1.80)	0.00 (0.00-2.00)	0.97 (1.30)	0.78
D4-Self-image/social interaction	0.00 (0.00-0.00)	0.38 (1.05)	0.00 (0.00-0.00)	0.15 (0.54)	0.30

Variable		OHRQoL

		Child subscale		D1-Symptoms		D2-Function		D3-Psychological		D4- Self-image/social interaction

		Median	p-value	Median	p-value	Median	p-value	Median	p-value	Median	p-value

		(Q1-Q3)		(Q1-Q3)		(Q1-Q3)		(Q1-Q3)		(Q1-Q3)
MTR (rs1805087)
Total group caries-active (n = 114)
Dominant model	GG + AG	2.00	0.33	0.00	0.13	2.00	0.62	0.00	0.312	0.00	0.58
	(n = 40)	(0.25–6.75)		(0.00–0.00)		(0.00–3.75)		(0.00–2.00)		(0.00–0.00)
	AA	4.00		0.00		2.00		0.00		0.00
	(n = 74)	(1.75–7.00)		(0.00–1.00)		(0.00–4.00)		(0.00–2.00)		(0.00–0.00)
Recessive model	GG	1.00	0.39	0.00	0.66	0.00	0.66	0.00	0.458	0.00	0.67
	(n = 5)	(0.00–8.00)		(0.00–0.50)		(0.00–5.00)		(0.00–2.00)		(0.00–1.00)
	AA + AG	4.00		0.00		2.00		0.00		0.00
	(n = 109)	(1.00–7.00)		(0.00–1.00)		(0.00–4.00)		(0.00–2.00)		(0.00–0.00)
Low severity caries–active (n = 84)
Dominant model	GG + AG	2.50	0.82	0.00	0.35	2.00	0.98	0.00	0.702	0.00	0.55
	(n = 30)	(0.00–7.25)		(0.00–0.00)		(0.00–4.00)		(0.00–2.25)		(0.00–0.00)
	AA	3.00		0.00		2.00		0.00		0.00
	(n = 54)	(1.00–6.00)		(0.00–1.00)		(0.00–3.00)		(0.00–2.00)		(0.00–0.00)
Recessive model	GG	1.00	0.50	0.00	0.77	0.00	0.80	0.00	0.477	0.00	0.74
	(n = 5)	(0.00–8.00)		(0.00–0.50)		(0.00–5.00)		(0.00–2.00)		(0.00–1.00)
	AA + AG	3.00		0.00		2.00		0.00		0.00
	(n = 79)	(1.00–6.00)		(0.00–0.00)		(0.00–3.00)		(0.00–2.00)		(0.00–0.00)
High severity Caries–active (n = 30)
Dominant model	GG + AG	2.00	0.05	0.00	0.17	2.00	0.31	0.00	0.152	0.00	1.00
	(n = 10)	(1.75–5.00)		(0.00–0.25)		(0.75–3.25)		(0.00–2.00)		(0.00–0.00)
	AA	4.50		0.00		4.00		1.50		0.00
	(n = 20)	(4.00–8.00)		(0.00–2.00)		(0.00–5.00)		(0.00–2.00)		(0.00–0.00)
Recessive model*	GG	1.00	–	0.00	–	0.00	–	0.00	–	0.00	–
	(n = 0)	(0.00–8.00)		(0.00–0.50)		(0.00–5.00)		(0.00–2.00)		(0.00–1.00)
	AA + AG	4.00		0.00		2.50		0.00		0.00
	(n = 30)	(2.00–8.00)		(0.00–1.00)		(0.75–4.25)		(0.00–2.00)		(0.00–0.00)
MTRR (rs1801394)
Total group caries–active (n = 94)
Dominant model	GG + AG	4.00	0.22	0.00	0.08	2.00	0.54	0.50	0.162	0.00	0.08
	(n = 61)	(2.00–7.00)		(0.00–0.00)		(0.00–4.00)		(0.00–2.00)		(0.00–0.00)
	AA	3.00		0.00		2.00		0.00		0.00
	(n = 33)	(1.00–5.50)		(0.00–1.00)		(0.00–3.50)		(0.00–2.00)		(0.00–0.00)
Recessive model	GG	5.00	0.36	0.00	0.17	3.00	0.15	1.00	0.288	0.00	0.63
	(n = 12)	(2.00–7.00)		(0.00–0.00)		(1.00–4.00)		(0.00–3.00)		(0.00–0.00)
	AA + AG	4.00		0.00		2.00		0.00		0.00
	(n = 82)	(1.00–7.00)		(0.00–1.00)		(0.00–4.00)		(0.00–2.00)		(0.00–0.00)
Low severity caries–active (n = 69)
Dominant model	GG + AG		0.36	0.00	0.23	2.00	0.82	1.00	0.167	0.00	0.14
	(n = 44)			(0.00–0.00)		(0.00–3.75)		(0.00–2.00)		(0.00–0.00)
	AA			0.00		2.00		0.00		0.00
	(n = 25)			(0.00–1.00)		(0.00–3.50)		(0.00–2.00)		(0.00–0.00)
Recessive model	GG		0.81	0.00	0.32	2.00	0.86	2.00	0.231	0.00	0.55
	(n = 9)			(0.00–0.00)		(0.50–3.50)		(0.00–3.50)		(0.00–1.00)
	AA + AG			0.00		2.00		0.00		0.00
	(n = 60)			(0.00–0.75)		(0.00–3.75)		(0.00–2.00)		(0.00–0.00)
High severity Caries–active (n = 25)
Dominant model	GG + AG		0.39	0.00	0.25	3.00	0.51	0.00	0.790	0.00	0.32
	(n = 17)			(0.00–0.50)		(0.00–4.50)		(0.00–2.00)		(0.00–0.00)
	AA			0.50		2.00		0.00		0.00
	(n = 8)			(0.00–1.75)		(0.25–3.50)		(0.00–2.00)		(0.00–0.00)
Recessive model	GG		0.33	0.00	0.83	4.00	0.08	0.00	0.503	0.00	0.59
	(n = 3)			(0.00–0.00)		(3.00–0.00)		(0.00–0.00)		(0.00–0.00)
	AA + AG			0.00		2.00		0.00		0.00
	(n = 22)			(0.00–1.25)		(0.00–4.00)		(0.00–2.00)		(0.00–0.00)

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[1] Corresponding Author: Lívia Azeredo Alves Antunes E-mail: liviaazeredo@gmail.com