Preparation and characterization of a novel antimicrobial film dressing for wound healing application

Gonçalves, Melissa Marques; Carneiro, Jaqueline; Justus, Barbara; Espinoza, Joel Toribio; Budel, Jane Manfron; Farago, Paulo Vitor; Paula, Josiane Padilha de

doi:10.1590/s2175-97902020000118784

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Brazilian Journal of Pharmaceutical Sciences

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Article • Braz. J. Pharm. Sci. 56 • 2020 • https://doi.org/10.1590/s2175-97902020000118784 copy

Preparation and characterization of a novel antimicrobial film dressing for wound healing application

Authorship SCIMAGO INSTITUTIONS RANKINGS

Antibacterial activity and good mechanical properties are some of the characteristics required for an appropriate film dressing. A novel polymer blend was developed for wound healing application. Twenty-four formulations using the polymers chitosan, poly(vinyl alcohol) and/or ɛ-Polylysine and the plasticizer glycerol were designed using factorial design and then the films were prepared by the casting/solvent evaporation method. Seventeen films were obtained among the twenty-four proposed formulations that were characterized by Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Mechanical properties, such as tensile strength (σ), elongation at break (ɛ) and Young’s modulus (Y) as well as antibacterial properties were determined. The best candidate was then further analyzed with regard to porosity, Water Vapor Transmission Rate (WVTR), swelling and cytotoxicity experiments. The results showed a film with semi-occlusive characteristics, good mechanical properties and no toxic. Incorporation of ɛ-Polylysine increased antibacterial activity against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria.

Keywords:
Chitosan; Poly(vinyl alcohol); ɛ-Polylysine; Semi-occlusive dressing

Independent variable	(-) (mL)	(+) (mL)
Chitosan solution 1.5% w/v	10	30
PVA solution 3% w/v	10	30
ɛ-Polylysinesolution 3% w/v	10	30
Glycerol	0.5	1

Formulation	C hitosan ( 1.5% w/v)	PVA (3 % w/v)	ɛ - Polylysine (3 % w/v)	Glycerol
FD01	10 mL	10 mL	-	0.5 mL
FD02	30 mL	10 mL	-	0.5 mL
FD03	10 mL	30 mL	-	0.5 mL
FD04	30 mL	30 mL	-	0.5 mL
FD05	10 mL	10 mL	-	1 mL
FD06	30 mL	10 mL	-	1 mL
FD07	10 mL	30 mL	-	1 mL
FD08	30 mL	30 mL	-	1 mL
FD09	10 mL	-	10 mL	0.5 mL
FD10	30 mL	-	10 mL	0.5 mL
FD11	10 mL	-	30 mL	0.5 mL
FD12	30 mL	-	30 mL	0.5 mL
FD13	10 mL	-	10 mL	1 mL
FD14	30 mL	-	10 mL	1 mL
FD15	10 mL	-	30 mL	1 mL
FD16	30 mL	-	30 mL	1 mL
FD17	10 mL	10 mL	10 mL	1 mL
FD18	30 mL	10 mL	10 mL	1 mL
FD19	10 mL	30 mL	10 mL	1 mL
FD20	30 mL	30 mL	10 mL	1 mL
FD21	10 mL	10 mL	30 mL	1 mL
FD22	30 mL	10 mL	30 mL	1 mL
FD23	10 mL	30 mL	30 mL	1 mL
FD24	30 mL	30 mL	30 mL	1 mL

Test	σ (N/mm² ± SD^* * Standard deviation calculated after three experiments )	ɛ (mm ± SD)	Y (N/mm² ± SD^* * Standard deviation calculated after three experiments )
FD01	1.06 ± 0.330	43.20 ± 15.456	2.44 ± 0.520
FD02	5.46 ± 0.928	134.71 ± 11.207	3.66 ± 0.162
FD03	1.73 ± 0.284	37.96 ± 11.165	7.24 ± 0.119
FD04	1.78 ± 0.539	31.31 ± 7.595	7.03 ± 1.901
FD05	0.90 ± 0.182	27.01 ± 6.485	2.68 ± 0.558
FD06	1.01 ± 0.258	40.79 ± 15.432	2.67 ± 0.219
FD07	0.93 ± 0.229	24.19 ± 1.623	5.32 ± 1.582
FD08	1.18 ± 0.376	37.36 ± 16.935	3.49 ± 0.204
FD09	1.42 ± 0.057	60.72 ± 9.784	2.28 ± 0.230
FD10	3.38 ± 1.411	115.06 ± 33.150	2.30 ± 0.255
FD14	1.04 ± 0.135	67.75 ± 5.621	1.52 ± 0.097
FD16	0.54 ± 0.160	33.88 ± 9.880	1.53 ± 0.268
FD17	0.21 ± 0.038	20.63 ± 3.115	0.90 ± 0.167
FD18	1.21 ± 0.565	64.02 ± 32.091	2.03 ± 0.233
FD20	0.88 ± 0.0640	60.78 ± 8.280	1.77 ± 0.174
FD22	1.03 ± 0.388	45.45 ± 24.384	2.95 ± 0.446
FD24	0.71 ± 0.182	54.39 ± 23.89	1.70 ± 0.178

Test	S. aureus	E. coli
FD01	6^* * 6mm is the diameter of the disc	6
FD02	6	6
FD03	6	6
FD04	6	6
FD05	6	6
FD06	6	6
FD07	6	6
FD08	6	6
FD09	12	12
FD10	10	10
FD14	10	10
FD16	12	12
FD17	10	12
FD18	10	10
FD20	12	12
FD22	10	10
FD24	12	10

Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Av. Prof. Lineu Prestes, n. 580, 05508-000 S. Paulo/SP Brasil, Tel.: (55 11) 3091-3824 - São Paulo - SP - Brazil
E-mail: bjps@usp.br

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[1] *Correspondence: Universidade Federal do Paraná. Av. Prefeito Lothário Meissner, 632. Jardim Botânico, Curitiba, Paraná, Brazil. Phone: +5541991750311E-mail address: melissamg1106@gmail.com