Cell phenotypes as activity biomarkers in patients with Systemic Lupus Erythematosus

The pathogenesis of systemic lupus erythematosus (SLE) is complex. Few studies in Brazilian population have addressed cell phenotypes associated with immunological responses and their associations with SLE activity. The aim of this study is to investigate cell phenotypes associated to SLE diagnosis, treatment and activity. Twenty-eight SLE female patients (17 inactive, 11 active) and 10 healthy women were included in this study. Markers of natural killer (Nk), T and B cells in peripheral blood were evaluated by flow cytometry. Nkt cells were decreased only in SLE active patients. Activated CD4+, regulatory T FoxP3+ and B cells were decreased in both active and inactive SLE patients, compared to control group. The data corroborate the disruption of immune regulatory response in SLE patients and suggest phenotipic changes as possible biomarkers of SLE activity.

Keywords:
Systemic Lupus Erythematosus; Lupus nephritis; T cells; B cells; NK cells

Authorship

Cristina de Mello Gomide Loures

conducted the experiments

drafted the manuscript

Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilFederal University of Minas GeraisBrazilBelo Horizonte, Minas Gerais, BrazilDepartment of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Tânia Mara Pinto Dabés Guimarães

handwriting the manuscript

Karine Silveste Ferreira

collected and processed the samples

Marcos Vinicius Ferreira Silva

collected and processed the samples

Luan Carlos Vieira Alves

collected and processed the samples

manuscript submission

https://orcid.org/0000-0002-9024-8872

Walter Batista Cicarini

collected and processed the samples

Fernanda Freire Campos Nunes

reviewed the cytometry analyzes

Renato Vargas Consoli

evaluation and recruitment of participants

Department of Rheumatology, Santa Casa, Belo Horizonte, Minas Gerais, BrazilSanta CasaBrazilBelo Horizonte, Minas Gerais, BrazilDepartment of Rheumatology, Santa Casa, Belo Horizonte, Minas Gerais, Brazil

Cláudia Lopes Santoro Neiva

evaluation and recruitment of participants

Paulo Madureira de Pádua

evaluation and recruitment of participants

Luara Isabela dos Santos

assisted in the standardization of antibodies

Oswaldo Cruz Foundation, Belo Horizonte, Minas Gerais, BrazilOswaldo Cruz FoundationBrazilBelo Horizonte, Minas Gerais, BrazilOswaldo Cruz Foundation, Belo Horizonte, Minas Gerais, Brazil

Josimar Dornelas Moreira

assisted in the statistical analysis

Vicente de Paulo Coelho Peixoto de Toledo ^**Correspondence: V. de P. C. P. de Toledo. Departmento de Análises Clínicas e Toxicológicas. Faculdade de Farmácia. Universidade Federal de Minas Gerais. R. Prof. Moacir Gomes de Freitas, Pampulha, Belo Horizonte, MG. CEP 31270-901, Belo Horizonte, MG, Brazil. Phone: +553134996876. E-mail: vpeixotoledo@yahoo.com.

purchase of reagents and handwriting

Maria das Graças Carvalho

writing of the manuscript

purchase of reagents

https://orcid.org/0000-0002-1669-3302

*Correspondence: V. de P. C. P. de Toledo. Departmento de Análises Clínicas e Toxicológicas. Faculdade de Farmácia. Universidade Federal de Minas Gerais. R. Prof. Moacir Gomes de Freitas, Pampulha, Belo Horizonte, MG. CEP 31270-901, Belo Horizonte, MG, Brazil. Phone: +553134996876. E-mail: vpeixotoledo@yahoo.com.

The authors of this study declare no relevant conflicts of interest.

SCIMAGO INSTITUTIONS RANKINGS

Oswaldo Cruz Foundation, Belo Horizonte, Minas Gerais, BrazilOswaldo Cruz FoundationBrazilBelo Horizonte, Minas Gerais, BrazilOswaldo Cruz Foundation, Belo Horizonte, Minas Gerais, Brazil

Figures

Figures (4)

Thumbnail

FIGURE 1
Phenotypic expression (%) comparisons of the population of CD3+CD4+, CD3+CD8+ and CD3+CD8+HLADR+ cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) (B) lupus nephritis and control group (NC). ANOVA, T-Student. Significance: p <0.05.

Thumbnail

FIGURE 2
Phenotypic expression (%) comparisons of CD3+CD4+CD25highFoxP3+ (regulatory) T cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) lupus nephritis (B) and control group (NC). ANOVA, T-Student. Significance: p <0.05.

Thumbnail

FIGURE 3
Phenotypic expression (%) comparisons of CD19+ B cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) lupus nephritis (B) and control group (NC). ANOVA, T-Student. Significance: p <0.05.

Thumbnail

FIGURE 4
Phenotypic expression (%) comparisons of CD3-CD56+, CD3-CD56+NKG2D+ (activated) and CD3+CD56+(NKt) NK cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) lupus nephritis (B) and control group (NC). ANOVA, T-Student. Significance: p <0.05.

FIGURE 1 Phenotypic expression (%) comparisons of the population of CD3+CD4+, CD3+CD8+ and CD3+CD8+HLADR+ cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) (B) lupus nephritis and control group (NC). ANOVA, T-Student. Significance: p <0.05.

FIGURE 2 Phenotypic expression (%) comparisons of CD3+CD4+CD25highFoxP3+ (regulatory) T cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) lupus nephritis (B) and control group (NC). ANOVA, T-Student. Significance: p <0.05.

FIGURE 3 Phenotypic expression (%) comparisons of CD19+ B cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) lupus nephritis (B) and control group (NC). ANOVA, T-Student. Significance: p <0.05.

FIGURE 4 Phenotypic expression (%) comparisons of CD3-CD56+, CD3-CD56+NKG2D+ (activated) and CD3+CD56+(NKt) NK cells among patients with inactive (SLE-I) or active (SLE-A) systemic lupus erythematosus (A), with (LN+) or without (no LN) lupus nephritis (B) and control group (NC). ANOVA, T-Student. Significance: p <0.05.

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Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Av. Prof. Lineu Prestes, n. 580, 05508-000 S. Paulo/SP Brasil, Tel.: (55 11) 3091-3824 - São Paulo - SP - Brazil
E-mail: bjps@usp.br

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Cell phenotypes as activity biomarkers in patients with Systemic Lupus Erythematosus

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[1] *Correspondence: V. de P. C. P. de Toledo. Departmento de Análises Clínicas e Toxicológicas. Faculdade de Farmácia. Universidade Federal de Minas Gerais. R. Prof. Moacir Gomes de Freitas, Pampulha, Belo Horizonte, MG. CEP 31270-901, Belo Horizonte, MG, Brazil. Phone: +553134996876. E-mail: vpeixotoledo@yahoo.com.