Figure 1
Morphology of SMMC-7721 cells under inverted microscope (100×). A, 2 g/L Fe2O3 MFH group; B, 4 g/L Fe2O3 MFH group; C, 6 g/L Fe2O3 MFH group; D, 8 g/L Fe2O3 MFH group; E, 8 g/L Fe2O3 spiking group (no radiation); F, control group A (RPMI 1640 culture medium only). MFH: magnetic fluid hyperthermia.
Figure 2
Morphology of SMMC-7721 cells after treatment. A, spiking group (8 g/L Fe2O3, no radiation); B-D, MFH groups (4, 6 and 8 g/L Fe2O3, respectively). MFH: magnetic fluid hyperthermia.
Figure 3
Energy spectrum of intracellular magnetic nanoparticle deposits in SMMC-7721 cells.
Figure 4
MTT histogram of SMMC-7721 cells after treatment. GR: growth rate; IR: inhibition rate; NCG: control group A (RPMI 1640 culture medium only); SMFG: control group B (RPMI 1640 culture medium and radiation); SMUHG: spiking group (8 g/L Fe2O3, no radiation); MFHG: magnetic fluid hyperthermia groups with Fe2O3 concentrations of 2, 4, 6, and 8 g/L, respectively. *P<0.05 compared with NCG growth rate; +P<0.05 compared with NCG inhibition rate (t-test).
Figure 5
Flow cytometry of SMMC-7721 cells after treatment. A, Control group A (RPMI 1640 culture medium only); B, spiking group (8 g/L Fe2O3, no radiation); C, 2 g/L Fe2O3 MFH group; D, 4 g/L Fe2O3 MFH group; E, 6 g/L Fe2O3 MFH group; F, 8 g/L Fe2O3 MFH group. MFH: magnetic fluid hyperthermia.
Figure 6
Apoptosis of SMMC-7721 cells (200×) after treatment. A, Control group (RPMI 1640 culture medium only); B, spiking group (8 g/L Fe2O3, no radiation); C, 2 g/L Fe2O3 MFH group; D, 4 g/L Fe2O3 MFH group; E, 6 g/L Fe2O3 MFH group; F, 8 g/L Fe2O3 MFH group. MFH: magnetic fluid hyperthermia.
Figure 7
Apoptosis rates of treatment groups. CG: control group, SMUHG: spiking group (8 g/L Fe2O3, no radiation), MFHG: magnetic fluid hyperthermia groups with 2, 4, 6, and 8 g/L Fe2O3. *P<0.05 compared with control and spiking group (t-test).
Figure 8
Immunochemical images (400×) of Hsp70 expression in SMMC-7721 hepatoma cells. A, Control group A (RPMI 1640 culture medium only); B, spiking group (8 g/L Fe2O3, no radiation), after 12 h; C, 2 g/L Fe2O3 MFH group, after 12 h; D, 4 g/L Fe2O3 MFH group, after 12 h; E, 6 g/L Fe2O3 MFH group, after 12 h; F, 8 g/L Fe2O3 MFH group, after 12 h. MFH: magnetic fluid hyperthermia.
Figure 9
p53 histogram of SMMC-7721 cells after treatment. CG: control group A; SMUHG: spiking group (8 g/L Fe2O3, no radiation); MFHG: MFH group; PI: positive index. *P<0.05 compared with CG (t-test).
Figure 10
Immunocytochemical images (200×) of p53 expression in SMMC-7721 hepatoma cells. A, Control group A (RPMI 1640 culture medium only); B, spiking group (8 g/L Fe2O3, no radiation); C, 2 g/L Fe2O3 MFH group; D, 4 g/L Fe2O3 MFH group; E, 6 g/L Fe2O3 MFH group; F, 8 g/L Fe2O3 MFH group. MFH: magnetic fluid hyperthermia.
Figure 11
Immunocytochemical images (400×) of Bax expression in SMMC-7721 hepatoma cells. A, Control group A (RPMI 1640 culture medium only); B, spiking group (8 g/L Fe2O3, no radiation); C, 2 g/L Fe2O3 MFH group; D, 4 g/L Fe2O3 MFH group; E, 6 g/L Fe2O3 MFH group; F, 8 g/L Fe2O3 MFH group. MFH: magnetic fluid hyperthermia.
Figure 12
Immunocytochemical images (400×) of Bcl-2 expression in SMMC-7721 hepatoma cells. A, Control group A (RPMI 1640 culture medium only); B, spiking group (8 g/L Fe2O3, no radiation); C, 2 g/L Fe2O3 MFH group; D, 4 g/L Fe2O3 MFH group; E, 6 g/L Fe2O3 MFH group; F, 8 g/L Fe2O3 MFH group. MFH: magnetic fluid hyperthermia.
Figure 13
Results of RNA electrophoresis of SMMC-7721 cells in 3 groups. CG: control group A (RPMI 1640 culture medium only); SMUHG: spiking group (8 g/L Fe2O3, no radiation); MFHG: magnetic fluid hyperthermia group (lane 3: 2 g/L Fe2O3; lane 4: 8 g/L Fe2O3).
Figure 14
RT-PCR detection of Hsp70 expression for SMMC-7721 cells after treatment for 12 h (A) and 24 h (B). NC: control group A (distilled water only); UHG: spiking group (8 g/L Fe2O3, no radiation); MFH4: MHF group with 8 g/L Fe2O3; MFH3: MHF group with 6 g/L Fe2O3; PC: control group B (MCF-7 cell group only); MFH2: MHF group with 4 g/L Fe2O3; MFH1: MHF group with 2 g/L Fe2O3.