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Veterinary Microbiology • Braz. J. Microbiol. 41 (3) • Oct 2010 • https://doi.org/10.1590/S1517-83822010000300034   copy

Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay

Authorship SCIMAGO INSTITUTIONS RANKINGS

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.

Bovine coronavirus; restriction enzyme; genotypes; gene S

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br
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