Preliminary studies of new strains of <i>Trametes</i> sp. from Argentina for laccase production ability

Fonseca, María Isabel; Tejerina, Marcos Raúl; Sawostjanik-Afanasiuk, Silvana Soledad; Giorgio, Ernesto Martin; Barchuk, Mónica Lucrecia; Zapata, Pedro Darío; Villalba, Laura Lidia

doi:10.1016/j.bjm.2016.01.002

Acessibilidade / Reportar erro

Brasil

Brazilian Journal of Microbiology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Environmental Microbiology • Braz. J. Microbiol. 47 (2) • Apr-Jun 2016 • https://doi.org/10.1016/j.bjm.2016.01.002 copy

Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu²⁺ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu²⁺. Strain B showed the greatest response to Cu²⁺ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.

Keywords
White rot fungi; Trametes; Laccase; Manganese peroxidase; Cu²⁺ response

	Laccase	MnP
	Enzymatic activity U mL⁻¹	Enzymatic activity U mL⁻¹
Strain A	190.74 ± 15.33	60.42 ± 29.32
Strain B	78.76 ± 12.74	37.44 ± 19.74
Strain C	325.00 ± 19.05	Undetectable
Strain E	19.65 ± 4.21	Undetectable

Time (min)	Residual enzymatic activity
	Strain A		Strain B		Strain C		Strain E
	With 0.5 mM of Cu²⁺	Without Cu²⁺	With 0.5 mM of Cu²⁺	Without Cu²⁺	With 0.5 mM of Cu²⁺	Without Cu²⁺	With 0.5 mM of Cu²⁺	Without Cu²⁺
0	100	100	100	100	100	100	100	100
20	91	113	82	92	63	65	81	92
40	55	97	58	61	52	47	69	32
60	59	96	31	58	43	54	36	7
120	56	51	36	13	31	12	23	8
180	52	49	32	14	30	–	18	–
240	48	36	27	–	17	–	10	–
300	56	32	29	–	13	–	10	–
360	40	31	26	–	13	–	6	–
420	45	–	24	–	–	–	6	–

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *Corresponding author. biotecmol2010@gmail.com