A study of the kinetics of denitrification was carried out in the laboratory based on the quantification of N2O, the final product of the activity of denitrifying microorganisms, when the enzymatic reduction of N2O to N2 was blocked by acetylene. Concentrated mixed liquor (sludge from a reactor with intermittent aeration used for sewage treatment) was used as the inoculum, while methanol, acetic acid, glucose, effluent sewage from an anaerobic fluidized bed reactor and synthetic substrate simulating domestic sewage were used as carbon sources. The mean concentration of nitrate was 20 mg/L. Maxima of N2O production and NO3- consumption occurred between 0.5h and 2.0h of incubation using all the carbon sources, which characterized the denitrification process. Acetic acid and methanol were responsible for the highest rates of N2O production. The estimated number of denitrifying microorganisms in the reactor with intermittent aeration, using the MPN technique, varied from 10(9) to 10(10) MPN/g VSS, indicating a high potential for the occurrence of denitrification.
denitrifying activity; denitrifying bacteria; denitrification; tertiary treatment of domestic sewage
