<i>Trypanosoma cruzi</i>: analysis of two different strains after piplartine treatment

Vieira, Gabriela Alves Licursi; Silva, Marco Túlio Alves da; Regasini, Luis Octávio; Cotinguiba, Fernando; Laure, Helen Julie; Rosa, José César; Furlan, Maysa; Cicarelli, Regina Maria Barretto

doi:10.1016/j.bjid.2018.02.009

Gabriela Alves Licursi Vieira

Universidade Estadual Paulista - UNESP, Instituto de Química, Araraquara, SP, Brazil

1 GALV and MTAS contributed equally to this manuscript.

Marco Túlio Alves da Silva ^**Corresponding authors. silvamta@gmail.com (M.T. Silva), cicarell@fcfar.unesp.br (R.M. Cicarelli).

Universidade de São Paulo, Instituto de Física de São Carlos, São Carlos, SP, Brazil

1 GALV and MTAS contributed equally to this manuscript.

Luis Octávio Regasini

Universidade Estadual Paulista - UNESP, Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Preto, SP, Brazil

Fernando Cotinguiba

Universidade Estadual Paulista - UNESP, Instituto de Química, Araraquara, SP, Brazil

Instituto de Pesquisas de Produtos Naturais (IPPN), Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil

Helen Julie Laure

Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Centro de Química de Proteínas, Ribeirão Preto, SP, Brazil

José César Rosa

Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Centro de Química de Proteínas, Ribeirão Preto, SP, Brazil

Maysa Furlan

Universidade Estadual Paulista - UNESP, Instituto de Química, Araraquara, SP, Brazil

Regina Maria Barretto Cicarelli ^**Corresponding authors. silvamta@gmail.com (M.T. Silva), cicarell@fcfar.unesp.br (R.M. Cicarelli).

Universidade Estadual Paulista - UNESP, Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brazil

*Corresponding authors. silvamta@gmail.com (M.T. Silva), cicarell@fcfar.unesp.br (R.M. Cicarelli).

Conflicts of interest

The authors declare no conflicts of interest.

1
GALV and MTAS contributed equally to this manuscript.

	Labeling samples
GEL 1	Pool (50 µg of proteins + 1 µL of Cy2)	Control 1 (50 µg of proteins + 1 µL of Cy3)	Treated 1 (50 µg of proteins + 1 µL of Cy5)
GEL 2	Pool (50 µg of proteins + 1 µL of Cy2)	Control 2 (50 µg of proteins + 1 µL of Cy5)	Treated 2 (50 µg of proteins + 1 µL of Cy3)
GEL 3	Pool (50 µg of proteins + 1 µL of Cy2)	Control 3 (50 µg of proteins + 1 µL of Cy3)	Treated 3 (50 µg of proteins + 1 µL of Cy5)
GEL 4	Preparative (gel to cut the spots) (500 µg of proteins of all samples applied in the experiment-control and treated triplicates)

Spot	X-fold differential expression		NCBI		MASCOT
	Control	Treated	Protein	Access number	Peptides	Mass (Da)	Score	pI	Amino acid sequence cover (%)
1	+2.111		Cytochrome C oxidase subunit IV	gi\|71660419	FTGEPPSWMR ACTDLNQLEEWSR	39,119	123	5.72	6.90
2	+2.194		Tryparedoxin peroxidase	gi\|71408703	ANEYFEK EAAPEWAGK VVQAFQYVDK HITVNDLPVGR DYGVLIEEQGISLR	25,734	338	5.96	22.60
3	+2.349		Tryparedoxin peroxidase	gi\|17224953	QITVNDLPVGR SYGVLKEEDGVAYR HGEVCPANWKPGDK	22,660	183	5.96	19.60
4	+2.483		Beta-tubulin	gi\|1220547	LREEYPDR LAVNLVPFPR FPGQLNSDLR KLAVNLVPFPR FPGQLNSDLRK INVYFDEATGGR AVLIDLEPGTMDSVR AVLIDLEPGTMDSVR GHYTEGAELIDSVLDVR	50,126	513	4.74	16.70
5	+2.853		Cystathionine beta-synthase	gi\|71404306	GYVLLDQYR SADKESFALASEVHR AHYEGTAQEIYDQCGK	47,830	308	6.43	9.60
6		+1.890	Methylthioadenosine phosphorylase	gi\|71655964	VGGVDCAFLPR TFPGLAAGTEGWR HGVGHVLNPSEVNYR LHEQGTSVTMEGPQFSTK LHEQGTSVTMEGPQFSTK YYAIETPFGRPSGDICVK	33,523	456	6.34	24.80
7		+1.951	Prostaglandin F2α synthase	gi\|25006239	YDFEEADQQIR ETYGVPEELTDDEVR AQQNWPLNEPRPETYR	42489	235	5.83	11.60
8		+1.962	Prostaglandin F2α synthase	gi\|25006239	FIANPDLVER YDFEEADQQIR KIEPLSLAYLHYLR ETYGVPEELTDDEVR AQQNWPLNEPRPETYR	42,489	391	5.83	17.90
9		+1.982	Heat shock 70 kDa protein, mitochondrial precursor	gi\|71407515	DAGTIAGLNVIR EISEVVLVGGMTR EISEVVLVGGMTR RFEDSNIQHDIK KVSNAVVTCPAYFNDAQR	71,503	242	5.75	8.40
10		+2.024	70 kDa heat shock protein	gi\|119394467	FEELCGDLFR AVHDVVLVGGSTR TTPSYVAFTDTER	72,428	238	5.37	5.40

Spot	X-fold differential expression		NCBI		MASCOT
	Control	Treated	Protein	Access number	Peptides	Mass (Da)	Score	pI	Amino acid sequence cover (%)
1	+1.310		Tryparedoxin peroxidase	gi\|17224953	QITVNDLPVGR SYGVLKEEDGVAYR HGEVCPANWKPGDK	22,660	219	5.96	19.60
2	+1.796		Methylthioadenosine phosphorylase	gi\|71655964	VGGVDCAFLPR TFPGLAAGTEGWR HGVGHVLNPSEVNYR YYAIETPFGRPSGDICVAK	33,523	398	6.34	18.90
3	+1.928		D-isomer specific 2-hydroxyacid dehydrogenase-protein	gi\|71420052	EVDEYGVR TANLVPDSVR FIGLVNEYAK TANLVPDSVRR RLDTYLVDPVR GITQNEDDLACALR ESDFVVNILPGTEETKR	38,795	479	6.04	20.90
4	+1.982		Tryparedoxin peroxidase	gi\|17224953	QITVNDLPVGR SYGVLKEEDGVAYR HGEVCPANWKPGDK	22,660	221	5.96	19.60
5	+2.144		Cystathionine beta-synthase	gi\|71404306	GYVLLDQYR SADKESFALASEVHR AHYEGTAQEIYDQCGGK TETALPWDHPDSLIGMAR	47,830	421	6.43	13.80
6		+1.529	Chaperonin HSP60, mitochondrial protein	gi\|3023478	NVIIEQSYGAPK GLIDGETSDYNR GYISPYFVTDAK AVGVILQSVAEQSR YVNMFEAGIIDPAR AAVQEGIVPGGGVALLR ALDSLLGDSSLTADQR VLENNDVTVGYDAQR KVTSTENIVQVATISANGDEELGR	59,639	801	5.38	24.20
7		+1.544	Heat shock 70 kDa protein, mitochondrial precursor	gi\|71407515	EISEVVLVGGMTR RFEDSNIQHDIK KVSNAVVTCPAYFNDAQR	71,503	184	5.75	6.60
8		+1.683	Heat shock protein 70	gi\|10626	TTPSYVAFTDTER ARFEELCGDLFR AVVTVPAYFNDSQR DCHLLGTFDLSGIPPAPR SQIFSTYADNQPGVHIQVFEGER	74,564	437	5.64	11.80
9		+1.864	Methylthioadenosine phosphorylase	gi\|71655964	VGGVDCAFLPR TFPGLAAGTEGWR HGVGHVLNPSEVNYR LHEQGTSVTMEGPQFSTK YYAIETPFGRPSGDICVAK	33,523	516	6.34	24.80
10		+2.570	Peptide methionine sulfoxide reductase	gi\|71405176	IHDPTTVNR QGVDVGTQYR AFGDAQVVTSLEK SAIFYHDDQQLK LHVAEDYHQMYLEK LHVAEDYHQMYLEK	20,204	386	6.12	32.80

[1] *Corresponding authors. silvamta@gmail.com (M.T. Silva), cicarell@fcfar.unesp.br (R.M. Cicarelli).

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Trypanosoma cruzi: analysis of two different strains after piplartine treatment

ABSTRACT