Heterotrophic cultivation of <i>Euglena gracilis</i> on chemically pretreated media

Pavlečić, Mladen; Crnić, Dijana; Jurković, Ena; Šantek, Mirela Ivančić; Rezić, Tonči; Šantek, Božidar

doi:10.1590/0104-6632.20180351s2016045

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Brazilian Journal of Chemical Engineering

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Bioprocess Engineering • Braz. J. Chem. Eng. 35 (1) • Jan 2018 • https://doi.org/10.1590/0104-6632.20180351s2016045 copy

Heterotrophic cultivation of Euglena gracilis on chemically pretreated media

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

In this research, the impact of chemical agents on the growth of Euglena gracilis and contaminants (S. cerevisiae and B. subtilis) in different cultivation media was studied. E. gracilis was cultivated on modified Hutner and complex medium in Erlenmeyer flasks and a stirred tank bioreactor. H₂O₂ and antimycin were used as suppressors of contaminant growth activities during algae cultivation. The use of antimycin as a chemical suppressor of contaminants is not recommendable because of its significant impact on the E. gracilis growth. At a H₂O₂ concentration of 5 mg L^-1 contaminant growth activities were almost completely suppressed. In these conditions, E. gracilis is capable to grow, but a further increase of H₂O₂ concentration is related to significant reduction of algae growth. H₂O₂ as a suppressor of contaminants has great potential for industrial application, but its optimal concentration for a particular bioprocess has to be determined in order to obtain the maximal bioprocess efficiency.

Keywords:
Euglena gracilis; heterotrophic cultivation; different cultivation media;H₂O₂; antimycin

H₂ O₂ concentration (mg L^-1 )	X_T (g L^-1 )	Y_X/S (g g^-1 )	Pr (g L^-1 h^-1 )
0	12.87 ± 0.62	0.660	0.107
1.5	6.75 ± 0.42	0.373	0.056
3	6.63 ± 0.32	0.368	0.055
4.5	7.30 ± 0.48	0.405	0.061
5	7.53 ± 0.28	0.560	0.063
6	7.07 ± 0.36	0.413	0.059
7.5	6.86 ± 0.34	0.387	0.057
9	5.97 ± 0.27	0.364	0.050
12	2.86 ± 0.22	0.062	0.024
15	1.26 ± 0.18	0.031	0.011

Antimycin concentration (g L^-1 )	X_T (g L^-1 )	Y_X/S (g g^-1 )	Pr (g L^-1 h^-1 )
0	12.32 ± 0.58	0.645	0.103
0.2	3.28 ± 0.27	0.196	0.027
0.3	2.71 ± 0.21	0.166	0.023
0.35	0.80 ± 0.12	0.050	0.007
0.5	0.14 ± 0.04	0.009	0.001
0.6	0.06 ± 0.01	0.003	0.0005
0.9	0	0	0
1.2	0	0	0

Cultivation system: A) Erlenmeyer flasks	X_T (g L^-1 )	Y_X/S (g g^-1 )	Pr (g L^-1 h^-1 )
CM	10.80 ± 0.54	0.600	0.090
CM + 5 mg L^-1 H₂O₂	7.94 ± 0.33	0.490	0.066
CM + 5 mg L^-1 H₂O₂ + S. cerevisiae + B. subtilis	8.01 ± 0.39	0.410	0.066
B) *Stirred tank bioreactor*
CM	10.74 ± 0.58	0.594	0.086
CM + S. cerevisiae + 5 mg L^-1 H₂O₂	7.40 ± 0.39	0.416	0.062
CM + B. subtilis + 5 mg L^-1 H₂O₂	6.95 ± 0.31	0.405	0.059

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[1] **Corresponding author: Božidar Šantek. E-mail: bsantek@pbf.hr. Phone:+385 1 4605 290, Fax: +385 1 4836 424