Feasibility of biohydrogen production by co-digestion of vinasse (sugarcane stillage) and molasses in an AnSBBR

Roberta Albanez

Mauá School of Engineering, Mauá Institute of Technology (EEM/IMT), Praça Mauá 1, CEP 09.580-900 , São Caetano do Sul, SP, Brazil.

São Carlos School of Engineering, University of São Paulo (EESC/USP), Av. Trabalhador São-Carlense 400, CEP 13.566-590 , São Carlos, SP, Brazil.

Giovanna Lovato

Mauá School of Engineering, Mauá Institute of Technology (EEM/IMT), Praça Mauá 1, CEP 09.580-900 , São Caetano do Sul, SP, Brazil.

São Carlos School of Engineering, University of São Paulo (EESC/USP), Av. Trabalhador São-Carlense 400, CEP 13.566-590 , São Carlos, SP, Brazil.

Suzana Maria Ratusznei

Mauá School of Engineering, Mauá Institute of Technology (EEM/IMT), Praça Mauá 1, CEP 09.580-900 , São Caetano do Sul, SP, Brazil.

Marcelo Zaiat

São Carlos School of Engineering, University of São Paulo (EESC/USP), Av. Trabalhador São-Carlense 400, CEP 13.566-590 , São Carlos, SP, Brazil.

José Alberto D. Rodrigues ^**Corresponding author: José Alberto D. Rodrigues. E-mail: rodrigues@maua.br

Mauá School of Engineering, Mauá Institute of Technology (EEM/IMT), Praça Mauá 1, CEP 09.580-900 , São Caetano do Sul, SP, Brazil.

Conditions	Concentration (mgCOD.L^-1 )	Cycle time (h)	AVOL (gCOD.L^-1 .d^-1 )	Influent Composition
Conditions	Concentration (mgCOD.L^-1 )	Cycle time (h)	AVOL (gCOD.L^-1 .d^-1 )	%S	%M	%V
1	3000	4	5.1	100	0	0
2	4000	4	6.9	100	0	0
3	3000	4	5.1	67	0	33
4	3000	4	5.1	0	100	0
5	4000	4	6.9	0	100	0
6	3000	4	5.1	0	67	33
7	3000	3	6.9	0	67	33
8	3000	3	6.9	0	25	75

Condition	C_CT,I (mgCOD.L^-1 )	_CT (%)	_CF (%)	AVOL_CT (gCOD.L^-1 .d^-1 )	ASOL_CT (gCOD.TVS^-1 .d^-1 )
1	2960 ± 276	16 ± 9	21 ± 9	5.1	5.3
2	4073 ± 216	17 ± 3	22 ± 5	7.1	7.4
3	3038 ± 103	17 ± 3	20 ± 2	5.6	3.0
4	3172 ± 137	22 ± 5	24 ± 3	5.5	5.9
5	4084 ± 141	24 ± 2	27 ± 2	7.1	7.7
6	3075 ± 92	19 ± 4	22 ± 3	5.4	1.0
7	3124 ± 194	19 ± 4	21 ± 4	7.3	2.6
8	3374 ± 321	15 ± 5	20 ± 6	7.8	3.5
Condition	C_ST,I (mgCarbohydrate.L^-1)	_ST (%)	_SF (%)	AVOL_ST (gCarbohydrate.L^-1.d^-1)	ASOL_ST (gCarbohydrate.TVS^-1.d^-1)
1	2986 ± 78	98 ± 1	99 ± 2	5.1	5.3
2	3397 ± 115	97 ± 1	97 ± 1	5.9	6.2
3	2129 ± 448	96 ± 1	97 ± 1	3.9	2.1
4	2423 ± 226	96 ± 1	96 ± 1	4.2	4.5
5	3198 ± 367	96 ± 1	97 ± 1	5.6	6.1
6	1501 ± 141	94 ± 1	95 ± 1	2.6	0.5
7	1433 ± 104	92 ± 2	94 ± 1	3.4	1.2
8	1326 ± 100	83 ± 2	84 ± 2	3.1	1.4

Condition	pH		TA (mgCaCO₃ .L^-1 )		TVA (mgHAc.L^-1 )
Condition	Influent	Effluent	Influent	Effluent	Influent	Effluent
1	8.1± 0.1	4.8 ± 0.3	382 ± 30	161 ± 33	21 ± 9	977 ± 108
2	8.1± 0.1	4.6 ± 0.1	427 ± 8	149 ± 33	16 ± 1	979 ± 34
3	5.8 ± 0.1	4.4 ± 0.1	170 ± 16	62 ± 11	352 ± 50	945 ± 35
4	7.3 ± 0.1	4.5 ± 0.2	154 ± 31	60 ± 12	76 ± 21	718 ± 47
5	7.1 ± 0.2	4.3 ± 0.1	168 ± 17	3 ± 1	85 ± 7	882 ± 74
6	5.6 ± 0.2	4.6 ± 0.1	181 ± 21	103 ± 36	421 ± 21	888 ± 73
7	5.3 ± 0.3	4.5 ± 0.1	151 ± 24	67 ± 31	413 ± 17	816 ± 32
8	5.6 ± 0.2	4.9 ± 0.1	191 ± 21	280 ± 40	559 ± 109	1162 ± 108

Condition	Ethanol	Acetic acid	Propionic acid	Butyric acid
Condition	(mmol.L^-1 )	(mmol.L^-1 )	(mmol.L^-1 )	(mmol.L^-1 )
1	17.3	10.5	0.9	2.6
2	18.8	12.9	1.0	2.8
3	5.1	9.0	1.4	2.1
4	1.1	6.3	0.6	1.3
5	2.1	7.2	0.8	2.0
6	0.4	5.6	1.0	1.2
7	0.3	7.5	1.1	1.4
8	1.5	6.5	4.1	1.5

Condition	n_H2	MPr	SMPr	MYAL_S,m	MYAL_C,m	MYRL_S,m	MYRL_C,m	V_G	%H₂	%CH₄	%CO₂
1	63.3	18.1	18.8	3.6	3.6	17.0	3.6	624	38	0	62
2	133.1	37.7	39.5	5.3	5.5	23.7	5.5	1286	39	0	61
3	29.5	8.3	4.5	1.5	2.1	7.4	2.2	361	31	4	65
4	10.8	3.2	3.4	0.6	0.9	2.4	0.9	293	16	13	71
5	27.5	7.9	8.7	1.1	1.6	4.1	1.7	445	23	12	65
6	5.8	1.7	0.3	0.3	0.7	1.4	0.7	198	11	14	75
7	13.0	3.8	1.4	0.5	1.2	2.5	1.3	233	16	14	70
8	0.0	0.0	0.0	0.0	0.0	0.0	0.0	131	0	0	100

Parameter	3	6	7
k' _1S (h ^-1 )	0.93	0.90	0.78
k _1HAC (h ^-1 )	3.83	1.03	5.17
k _5HAC (h ^-1 )	0.00	0.55	0.00
k _6HAC (h ^-1 )	0.00	0.63	3.70
k _7HAC (h ^-1 )	0.00	0.11	0.87
k _2HPR (h ^-1 )	0.51	0.77	2.28
k _5HPR (h ^-1 )	0.00	0.21	0.44
k _3HBU (h ^-1 )	1.12	0.67	0.66
k _6HBU (h ^-1 )	0.00	0.18	0.04
k _4ETOH (h ^-1 )	2.79	0.04	0.17
k _1H (h ^-1 )	0.76	0.32	0.84
k _2H (h ^-1 )	0.05	0.68	1.16
k _3H (h ^-1 )	0.78	0.34	0.76
k _5H (h ^-1 )	0.00	0.03	0.00
k _6H (h ^-1 )	0.00	0.12	0.61
k _8H (h ^-1 )	0.15	0.98	2.10
k _7M (h ^-1 )	0.00	0.00	0.00
k _8M (h ^-1 )	0.07	0.43	0.77

{R V O L}_{S F} = \frac{(V_{F} . N) . (C_{S T, I} - C_{S F})}{V_{R}} {R V O L}_{C F} = \frac{(V_{F} . N) . (C_{C T, I} - C_{C F})}{V_{R}}

{R S O L}_{S F} = \frac{(V_{F} . N) . (C_{S T, I} - C_{S F})}{M_{T V S}} {R S O L}_{C F} = \frac{(V_{F} . N) . (C_{C T, I} - C_{C F})}{M_{T V S}}

{M Y R L}_{S, m} = \frac{n_{H 2}}{(N . V_{F}) . (C_{S T, I} - C_{S F})} {M Y R L}_{C, m} = \frac{n_{H 2}}{(N . V_{F}) . (C_{C T, I} - C_{C F})}

\frac{d C_{H}}{d t} = r_{H} = k_{1 H} \cdot C_{S} - k_{2 H} \cdot C_{S} + k_{3 H} \cdot C_{S} + k_{5 H} \cdot C_{H P r} + k_{6 H} \cdot C_{H B u} - k_{8 H} \cdot C_{H}

Brazil