UTILIZATION OF AGROINDUSTRIAL BY-PRODUCTS AS SUBSTRATE IN ENDOGLUCANASE PRODUCTION BY <i>Streptomyces diastaticus</i> PA-01 UNDER SUBMERGED FERMENTATION

Bispo, A.S.R.; Andrade, J.P.; Souza, D.T.; Teles, Z.N.S.; Nascimento, R.P.

doi:10.1590/0104-6632.20180352s20160415

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Bioprocess Engineering • Braz. J. Chem. Eng. 35 (2) • Apr-Jun 2018 • https://doi.org/10.1590/0104-6632.20180352s20160415 copy

UTILIZATION OF AGROINDUSTRIAL BY-PRODUCTS AS SUBSTRATE IN ENDOGLUCANASE PRODUCTION BY Streptomyces diastaticus PA-01 UNDER SUBMERGED FERMENTATION

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Endoglucanase production under submerged fermentation was studied using sugarcane bagasse (SCB) and oat bran (OB) as carbon source and corn steep liquor (CSL) as nitrogen source, in different concentrations using factorial design. Streptomyces diastaticus PA-01, isolated from a soil cave in Brazil, was selected as cellulolytic strain. The results after experimental validation showed that a medium containing 2.4% (w/v) SCB and 1.3% (w/v) CSL led to the highest production, 1,180.3 U.L^-1 of endoglucanase, after the 5^th-day. A good level of endoglucanase (1,039.3 U.L^-1) was obtained after the 4^th-day when 2.0% (w/v) OB and 1.65% (w/v) CSL were used. The pH and temperature profiles showed thermoacidophilic endoglucanase activity, with 70% of maximum activity at 50ºC, after 4 hours of pre-incubation. This is the first report on endoglucanase production by S. diastaticus PA-01 in the presence of SCB and OB. The strong positive effects of some metal ions (Zn²⁺, Mn²⁺ and Ba²⁺) on endoglucanase activity when this strain was grown on OB is an interesting biochemical characteristic for future biotechnological applications.

Keywords:
Streptomyces diastaticus PA-01; endoglucanase; oat bran; sugarcane bagasse; corn steep liquor

Run	Coded setting levels		Actual leves ( % w/v )		Endoglucanase activity (U.L^-1) OB – 4 days	Endoglucanase activity (U.L^-1) SCB – 5 days
Run	X₁	X₂	X₁	X₂	OV	OV
1	-1	-1	0.80	0.30	138.10	352.32
2	+1	-1	2.40	0.30	399.75	393.53
3	-1	+1	0.80	1.30	449.18	604.92
4	+1	+1	2.40	1.30	485.52	711.98
5	-1.41	0	0.47	0.80	188.98	438.74
6	+1.41	0	2.73	0.80	238.04	701.98
7	0	-1.41	1.60	0.10	280.55	186.13
8	0	+1.41	1.60	1.51	617.80	740.53
9	0	0	1.60	0.80	386.67	672.73
10	0	0	1.60	0.80	385.22	678.05
11	0	0	1.60	0.80	373.59	707.48

	Source of variations	Sum of squares	Degrees of freedom	Mean square	F-value	p-value^* * Statistically significant at 95% of confidence level. R 2 = 0.96, F4;6;0,05 = 4.53 (SCB), R2 = 0.95, F5;5;0,05 = 5.05 (OB)
SCB + CSL	Regression	337784.4	4	84446.1	36.9	< 0.05
	Residue	13731.2	6	2288.5	36.9	< 0.05
	Total SS	351515.6	10
OB + CSL	Regression	183286.8	5	36657.36	17.26	< 0.05
	Residue	10621.6	5	2124.32		< 0.05
	Total SS	193908.4	10

Ion ^a a The final concentration in the reaction mixture was 10 mM.	SCB + CSL	OB + CSL
	Relative activity (%)^b b Relative activity is expressed as a percentage of control	Relative activity (%) ^b b Relative activity is expressed as a percentage of control
Control (no addition)	100.00	100.00
Zn⁺²	62.96	185.39
Ba⁺²	44.98	179.61
Fe⁺²	29.51	141.17
K⁺	33.87	172.12
Mn⁺²	61.17	211.58
Co⁺²	37.22	174.85
Na⁺	30.53	152.39
Ca⁺²	3.35	166.00
Cu⁺²	1.79	64.97
Mg⁺²	24.97	162.94
EDTA	29.33	143.55

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[1] * Corresponding author: Rodrigo Pires do Nascimento - Dep. Engenharia Bioquímica, Escola de Química, Bloco E, sala E-108, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ - Brazil, 21941-909. Phone: + 55 21 3938-8863. e-mail: rodrigopires@eq.ufrj.br