ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY   <i>Trichoderma harzianum</i> P49P11

Mariño, M. A.; Freitas, S.; Miranda, E. A.

doi:10.1590/0104-6632.20150322s00003268

Acessibilidade / Reportar erro

Brasil

Brazilian Journal of Chemical Engineering

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

BIOPROCESS ENGINEERING • Braz. J. Chem. Eng. 32 (2) • Apr-Jun 2015 • https://doi.org/10.1590/0104-6632.20150322s00003268 copy

ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY Trichoderma harzianum P49P11

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

This study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regard less of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as FPase was 77% by precipitation carried out at this same pH and ethanol concentration but at 5.0 °C. Therefore, ethanol precipitation can be considered to be an efficient technique for xylanase concentration and, to a certain extent, also for the cellulase complex.

Keywords
Trichoderma harzianum; Glycosyl hydrolases; Ethanol precipitation

pH of the fermented broth	Temperature (°C)	Ethanol % (v/v)	Protein mass of the precipitate phase (%)	Protein mass of the supernatant phase (%)	Total recovery (%)
5.0	5.0	60	36.4 ± 4.9	57.6 ± 3.7	94.0
		75	81.7 ± 1.1	15.1 ± 2.7	96.8
		90	94.2 ± 2.4	10.6 ± 4.2	105
	15.0	60	31.2 ± 4.7	73.3 ± 1.4	104
		75	76.1 ± 5.4	10.6 ± 3.3	86.7
		90	85.5 ± 1.9	13.7 ± 6.1	99.2
	25.0	60	36.3 ± 4.5	48.7 ± 1.7	85.0
		75	63.7± 1.8	20.6± 6.9	84.3
		90	85.2 ± 2.2	13.5± 5.1	98.7
6.5	5.0	60	30.0 ± 2.7	60.0 ± 7.4	90.0
		75	80.1 ± 3.2	19.1 ± 3.3	99.2
		90	95.7 ± 3.6	6.50 ± 0.5	102
	15.0	60	34.9 ± 7.9	53.8 ± 7.3	88.7
		75	84.0 ± 16	16.5 ± 7.1	100
		90	90.1 ± 14	10.8 ± 1.8	101
	25.0	60	34.4 ± 1.0	51.4 ± 9.4	85.8
		75	59.3 ± 4.6	34.8 ± 4.0	94.1
		90	71.4 ± 2.4	21.5 ± 9.7	92.9
8.0	5.0	60	36.4 ± 2.3	44.3 ± 5.4	80.7
		75	75.2 ± 1.9	23.9 ± 5.9	99.1
		90	93.9 ± 1.9	6.70 ± 2.2	101
	15.0	60	33.7 ± 10	50.7 ± 1.9	84.4
		75	58.7 ± 13	46.6 ± 4.6	105
		90	83.7 ± 1.1	12.8 ± 3.2	96.5
	25.0	60	25.4 ± 1.3	48.7 ± 5.2	74.1
		75	51.2 ± 4.8	43.4 ± 6.8	94.6
		90	73.0 ± 10	27.3 ± 2.6	100

Brazilian Society of Chemical Engineering Rua Líbero Badaró, 152 , 11. and., 01008-903 São Paulo SP Brazil, Tel.: +55 11 3107-8747, Fax.: +55 11 3104-4649, Fax: +55 11 3104-4649 - São Paulo - SP - Brazil
E-mail: rgiudici@usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] * To whom correspondence should be addressed