Pouteria ramiflora leaf extract on emulgel in wound healing activity in diabetic rats

Ação do extrato de folhas de Pouteria ramiflora em emulgel na atividade de cicatrização de feridas em ratos diabéticos

Abstract

The genus Pouteria has been studied because it presents various activities, among which is its anti-inflammatory potential. The effects of Pouteria ramiflora Carbopol gel on the healing of skin wounds in diabetic rats were evaluated by microscopic imaging. Streptozotocin was administered intraperitoneally in animals that had fasted for 12 hours, a situation confirmed by the glycemic index (˃ 240 mg dL^-1). An excision on the back of the animals was performed and three groups were formed: Control (Gel), Ethanolic extract (Ext) and Gel + extract 2% (Ext+gel); the histopathological evaluation occurred on the 7^th, 14^th, 21^st and 30^th days after the post-operative period. The results of the phytochemical prospecting of P. ramiflora extract demonstrated the major presence of phenolic compounds and flavonoids; the assessment of the inflammatory infiltrate on the 7^th day was higher on group Ext and Ext+gel when compared to group Control; on the 14th day control and Ext (p<0.05). The quantification of fibroblasts was higher on the 7^th day among the three treatments, control and Ext (p<0.05), on the 21^st day. Angiogenesis showed a higher number of vessels in Ext+gel group (p<0.05) on the 7^th day; in Control, Ext and Ext+gel (p<0.05) on the 14^th day; and Control and Ext (p<0.05)on the 21^st day. The histopathological results showed that the formulation Ext+gel was efficient in tissue reparation and decrease in inflammatory cells on the diabetic’s animals.

Keywords:
Diabetes mellitus; tissue repair; healing plants; flavonoids