Prevalência de parasitas de <i>Lernaea</i> em espécies comerciais de peixes de água doce em várias pisciculturas no Paquistão

Bilal, M.; Abbas, F.; Atique, U.; Rehman, M. Hafeez-ur; Inayat, M.; Zohaib, M.; Saleem, M.; Fatima, S.; Sherazi, S. W. S. M.; Tehreem, A.; Ali, A.; Sanwal, M. U.; Abdullah, M.; Ullah, M.; Mubeen, N.

M. Bilal ^**e-mail: muhammadbilallive786@gmail.com; farzana.abbas@uvas.edu.pk; physioatique@gmail.com

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

University of Veterinary and Animal Sciences – UVAS, Institute of Biochemistry and Biotechnology, Lahore, Pakistan

http://orcid.org/0000-0001-8172-4729

F. Abbas ^**e-mail: muhammadbilallive786@gmail.com; farzana.abbas@uvas.edu.pk; physioatique@gmail.com

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

http://orcid.org/0000-0002-9036-2050

U. Atique ^**e-mail: muhammadbilallive786@gmail.com; farzana.abbas@uvas.edu.pk; physioatique@gmail.com

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

Chungnam National University, Department of Bioscience and Biotechnology, Daejeon, South Korea

http://orcid.org/0000-0001-9178-8443

M. Hafeez-ur Rehman

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

http://orcid.org/0000-0002-6609-4244

M. Inayat

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

http://orcid.org/0000-0002-6302-6422

M. Zohaib

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

University of Lahore, Sargodha Campus, Department of Zoology, Sargodha, Pakistan

http://orcid.org/0000-0002-0256-6433

M. Saleem

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

http://orcid.org/0000-0001-6299-3325

S. Fatima

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

University of Veterinary and Animal Sciences – UVAS, Institute of Biochemistry and Biotechnology, Lahore, Pakistan

http://orcid.org/0000-0002-3815-9119

S. W. S. M. Sherazi

University of Veterinary and Animal Sciences – UVAS, Institute of Biochemistry and Biotechnology, Lahore, Pakistan

http://orcid.org/0000-0003-4798-9410

A. Tehreem

University of Agriculture, Department of Zoology, Wildlife and Fisheries, Faisalabad, Pakistan

http://orcid.org/0000-0002-0653-1983

A. Ali

Chinese Academy of Sciences, Chengdu Institute of Biology, China

http://orcid.org/0000-0002-7646-6304

M. U. Sanwal

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

http://orcid.org/0000-0002-6850-5473

M. Abdullah

University of Veterinary and Animal Sciences – UVAS, Institute of Biochemistry and Biotechnology, Lahore, Pakistan

http://orcid.org/0000-0002-1488-2978

M. Ullah

Islamia College, Zoology Department, Peshawar, Pakistan

http://orcid.org/0000-0001-7077-0615

N. Mubeen

University of Veterinary and Animal Sciences – UVAS, Department of Fisheries and Aquaculture, Lahore, Pakistan

http://orcid.org/0000-0001-9305-4080

*e-mail: muhammadbilallive786@gmail.com; farzana.abbas@uvas.edu.pk; physioatique@gmail.com

Fish Farm	Size	Water source	Feed	% Feed given	Fertilizer	Stocking density/acre	Fish Species	Lernaea infestation	Treatment used
Royal Fish Farm, UVAS, Pattoki	2 acres	Tube well	AMG Fish Feed 22% CP	2%	Manure, Urea	1000	Rahu, Mori, Thella, Silver, Grass	84.3	Liming, KMnO₄
Integrated Aquaculture Fish Farms, UVAS, Pattoki	1 acre	Tube well	Supreme Feed (25% CP)	2%	Duck droppings, Urea	800	Rahu, Mori, Grass carp	8.8	Organophosphates
Mehtabi Lake Change Manga	8 acres	Tube well	Rice polish, Wheat bran, Soya been	2%	DAP, Urea	2000	Rahu, Mori, Thella, Grass carp, Silver, Hybrid (Rahu + Thella)	7.1	Liming, KMnO₄
Vincent’s Fish Farm	3 acres	Tube well	Forage / Fodder	2-4%	DAP, Urea, Manure	800	Grass carp, Rahu, Thella	0	Liming
Arain Fish Farm, Kasur	4 acres	Tube well	Rice police	1.5-2.5%	DAP, Urea, Manure	1000	Rahu, Thella, Mori, Grass carp, Common carp	7.3	Liming
Chunian Fish Farm, Kasur	1 acre	Rain, Canal water, Tube well	Forage, Husk, Cow dung, Fertilizer, Rice polish	2-4%	Manure	1200	Tilapia	0	Liming
Shahid Fish Farm, Kasur	2 acres	Tube well, Canal water	Rice polish, Maize gluten, Soya been	2%	DAP, Urea, Manure	1000	Rahu, Thella, Mori, Grass carp	11.7	No treatment measures taken

Fish farm	Vegetation	WT (°C)	pH	DO (mg/L)	BOD	TSS (mg/L)	Salinity (%)
Royal Fish Farm, UVAS, Pattoki	Grasses, Weeds, Algae, Fungi	14±19	8.9±1.0	7.5±1.4	5.8±0.2	10.0±0.3	10.0±0.2
Integrated Aquaculture Ponds UVAS, Pattoki	Algae, Grasses	15±15	7.5±1.5	7.1±0.4	4.2±0.1	8.0±0.7	6.5±0.4
Mehtabi Lake, Change Manga	Algae	23±10	7.3±1.1	6.3±0.7	3.9±0.1	7.1±0.9	7.9±0.3
Vincent’s Fish Farm, Kasur	Algae, Weeds	15±14	6.9±1.3	7.2±0.7	3.0±.3	4.0±1.3	4.5±0.5
Arain Fish Farm, Kasur	Grasses, Algae	19±15	7.1±0.9	6.4±1.4	3.7±0.1	5.5±0.9	5.6±0.9
Chunian Fish Farm, Kasur	Algae	12±16	7.0±1.5	6.5±05	2.9±0.4	3.2±0.6	4.0±0.1
Shahid Fish Farm, Kasur	Grasses, Algae, Weeds	16±15	8.7±1.3	6.8±0.6	3.5±0.3	6.0±0.9	8.3±0.8

Fish Farm	Samples	Samples infected	% Prevalence
Royal Fish Farm, UVAS, Pattoki	89	75	84.3
Integrated Aquaculture Ponds UVAS, Pattoki	57	5	8.8
Mehtabi Lake, Change Manga	70	5	7.1
Vincent’s Fish Farm, Kasur	47	0	0
Arain Fish Farm, Kasur	702	51	7.3
Chunian Fish Farm, Kasur	105	0	0
Shahid Fish Farm, Kasur	94	11	11.7
Total	1164	147	12.6

Fish species	Examined Samples	Infected Samples	Prevalence (%)
Labeo rohita	323	24	7.4
Ctenopharyngodon idella	422	58	13.2
Cirrhinus cirrhosis	37	3	8.1
Catla catla	144	60	41.7
Hypophthalmichthys molitrix	107	1	0.9
Cyprinus carpio	26	2	7.7
Oreochromis niloticus	105	0	0
Total	1164	147	12.6

Weight and Length categories	L. rohita	C. idella	C. cirrhosis	C. catla	H. molitrix	C. carpio	Total prevalence (%)
≤500g 10-20cm	7.1% 0%	27.8% 7%	50% -	16.7% 16.7%	0% 0%	50% -	13.3% 2.9%
501-1000g 21-30cm	4.2% 11.1%	29.7% 41.5%	9.1% 50%	5.9% 9.1%	0% 0%	0% 50%	12.7% 20%
1001-1500g 31-40cm	0% 0.8%	5.2% 28.8%	6.7% 6.7%	0% 0%	0% 0%	- 0%	2.6% 8.1%
1501-2000g 41-50cm	2.7% 1.8%	0% 2.9%	0% 9.1%	0% 6.7%	0% -	0% -	0.6% 2.9%
2001-2500g 51-60cm	22.2% 13.3%	0% 1.4%	0% 0%	33.3% 29.4%	0% 0%	5.3% 0%	8.9% 8.4%
2501-3000g 61-70cm	60% 50%	4.1% 50%	- 0%	63.3% 85%	0% 0%	0% 5.9%	30% 25.2%
3001-3500g 71-80cm	42.9% 57.1%	12% 21.2%	- -	84.6% 76.5%	0% 20%	- 0%	39.4% 39.7%
3501-4000g 81-90cm	75% 66.7%	38.5% -	- -	88.9% -	25% -	- -	56.7% 66.7%
Total	7.4%	13.7%	8.1%	41.7%	0.9%	7.7%

Body part	L. rohita (%)	C. idella (%)	C. cirrhosis (%)		C. catla (%)	H. molitrix (%)	C. carpio (%)	Total (%)	% Of 12.6% infestation
Lateral line	5 (0.4)	1 (0.1)		1 (0.1)	1 (0.1)	-	-	8 (0.7)	5.4
Dorsal fin	6 (0.5)	15 (1.3)		2 (0.2)	3 (0.3)	1 (0.1)	-	27 (2.3)	18.4
Ventral body plane	-	5 (0.4)	-		2 (0.2)	-	-	7 (0.6)	4.8
Ventral, dorsal and lateral body plane	4 (0.3)	9 (0.8)	-		11 (0.9)	-	1 (0.1)	25 (2.1)	17
Caudal region	1 (0.1)	10 (0.9)	-		2 (0.2)	-	1 (0.1)	14 (1.2)	9.5
Head	-	1 (0.1)	-		-	-	-	1 (0.1)	0.7
Pectoral fin	1 (0.1)	4 (0.3)	-		1 (0.1)	-	-	6 (0.5)	4.1
Pelvic fin	-	4 (0.4)	-		-	-	-	5 (0.4)	3.4
Snout	1 (0.1)	-	-		-	-	-	1 (0.1)	0.7
Whole fish	5 (0.4)	8 (0.7)	-		32 (2.7)	-	-	45 (3.9)	30.6
Eyes	-	0	-		5 (0.4)	-	-	5 (0.4)	3.4
Mouth	-	0	-		3 (0.3)	-	-	3 (0.3)	2
Total	2.1%	5%	0.3%		5.2%	0.1%	0.2%	147 (12.6)	-
% Within 12.6% infestation	15.6	39.5	2		40.8	0.7	1.4	-	100

Study Period	Fish species	Examined samples	Infected samples	Prevalence %	Percentage within 12.6 prevalence
Aug-Sep.
	Rahu,	21	1	4.8%
	Grass carp	15	2	13.3%
	Total	36	3	8.3%	2%
Oct-Nov.
	Rahu	40	2	5%
	Grass carp	27	1	3.7%
	Mrigal carp	5	0	0%
	Thella	13	0	0%
	Silver	5	0	0%
	Total	90	3	3.3%	2%
Dec-Jan.
	Rahu	262	21	8%
	Grass carp	380	55	14.5%
	Mrigal carp	32	3	9.4%
	Thella fish	131	60	45.8%
	Silver carp	1021	1	1%
	Tilapia	105	0	0%
	Common carp	26	2	7.7%
	Total	1038	142	13.7%	95.9%
Overall		1164	147	12.6%

[1] *e-mail: muhammadbilallive786@gmail.com; farzana.abbas@uvas.edu.pk; physioatique@gmail.com

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Prevalência de parasitas de Lernaea em espécies comerciais de peixes de água doce em várias pisciculturas no Paquistão

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