Amelioration potential of <i>Moringa oleifera</i> extracts against sodium arsenate induced embryotoxicity and genotoxicity in mouse (<i>Mus musculus</i>)

Ali, K.; Iqbal, A.; Bukhari, S. M.; Safdar, S.; Raiz, A.; Ali, W.; Hussain, A.; Javid, A.; Hussain, M.; Ali, M. M.; Mahmud, A.; Iqbal, M. J.; Nasir, M. F.; Mubeen, I.; Kanwal, S.; Sughra, F.; Khattak, A.; Saleem, M.

doi:10.1590/1519-6984.248022

K. Ali ^**e-mail: asia.iqbal@uvas.edu.pk

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0003-1153-8589

A. Iqbal

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-4394-6141

S. M. Bukhari

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-6330-9676

S. Safdar

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-2120-4104

A. Raiz

Department of Zoology, GC Women University, Faisalabad, Pakistan

http://orcid.org/0000-0002-7801-7481

W. Ali

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-4074-9898

A. Hussain

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-8105-3029

A. Javid

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-8499-7674

M. Hussain

University of Veterinary & Animal Sciences, Department of Wildlife & Ecology, Lahore, Pakistan

http://orcid.org/0000-0002-4017-588X

M. M. Ali

University of Veterinary & Animal Sciences, Institute of Biochemistry and Biotechnology, Lahore, Pakistan

http://orcid.org/0000-0001-7930-7782

A. Mahmud

University of Veterinary & Animal Sciences, Department of Poultry Production, Lahore, Pakistan

http://orcid.org/0000-0002-2106-4113

M. J. Iqbal

Bahauddin Zakariya University, Department of Zoology, Multan, Pakistan

http://orcid.org/0000-0001-8390-8476

M. F. Nasir

Bahauddin Zakariya University, Department of Zoology, Multan, Pakistan

http://orcid.org/0000-0002-9446-9234

I. Mubeen

Government College University, Department of Zoology, Lahore, Pakistan

http://orcid.org/0000-0003-4154-5296

S. Kanwal

University of Okara, Department of Zoology, Okara, Pakistan

http://orcid.org/0000-0001-7958-1327

F. Sughra

University of Education, Department of Zoology, Division of Science & Technology, Lahore, Pakistan

http://orcid.org/0000-0002-4470-8196

A. Khattak

Department of Bioinformatics, Shaheed Benazir Bhutto Women University, Peshawar, Pakistan

http://orcid.org/0000-0001-9694-3211

M. Saleem

Department of Zoology, Virtual University of Lahore, Lahore, Pakistan

http://orcid.org/0000-0003-4553-7496

*e-mail: asia.iqbal@uvas.edu.pk

Phytochemicals and Test	Leaves extract	Flowers extract
Mayers Test for Alkolides	++	+
H₂SO₄ test for Flavonoids	++	+
Liebermann Burchard test for Steroids	+	+
Salkowski test for Terpenoids	+	+
Ferric Chloride test for Phenols	+++	+
Frothing test Saponins	++	-
Ferric Chloride test for Tanins	+	+
Nitroprusside test for Glycosides	+	+
Ninhydrin test for Protein	+	+
Molisch test for Carbohydrates	++	+

Treatment Groups	Doses (mg/kg/B.w)	Maternal IBW (Mean ± SD)	Maternal FBW (Mean ± SD)
Control (A)	-	25 ± 0.81	44 ± 1.15^a
Sodium arsenate(B)	6	23.2 ± 0.95	30.5 ± 1.29^g
Sodium arsenate(C)	12	25 ± 0.81	30 ± 0.81^g
SA+MOFE (D)	6+150	23.2 ± 0.95	33.2 ± 0.50^f
SA+MOFE (E)	6+300	23.2 ± 0.95	30.7 ± 0.95^g
SA+MOFE (F)	12+150	24.7 ± 0.95	35.5 ± 0.57^e
SA+MOFE (G)	12+300	23 ± 0.81	35.7 ± 0.95^e
SA+MOLE (H)	6+150	24.2 ± 0.50	38.2 ± 0.95^d
SA+MOLE (I)	6+300	23.2 ± 0.95	42.2 ± 0.50^b
SA+MOLE (J)	12+150	24.7 ± 0.95	39.2 ± 0.50^d
SA+MOLE (K)	12+300	23.5 ± 1.29	40.5 ± 0.57^c

Doses groups mg/kg/B.W	Fetuses weight (mg)	Hind limb (mm)	Fore limb (mm)	Tail length (mm)	Snout length (mm)	Crown rump length (mm)	Head circumference (mm)
(A) Control	1330.12 ± 16.03^a	10.13 ± 0.46^a	9.26 ± 0.44^a	13.49 ± 0.19^a	4.48 ± 0.20^a	15.38 ± 0.16^a	21.52 ± 0.81^a
(B) SA 6 mg	530.64 ± 16.67^g	5.58 ± 0.15^hi	4.45 ± 0.17^g	6.54 ± 0.36ⁱ	3.40 ± 0.19^f	8.01 ± 0.11ⁱ	13.07 ± 0.08^h
(C) SA 12 mg	494.08 ± 54.56^h	5.51 ± 0.13^b	4.44 ± 0.14^g	6.47 ± 0.25ⁱ	3.37 ± 0.15^f	7.98 ± 0.07ⁱ	13.06 ± 0.09^h
(D) SA+MOFE 6 mg+150 mg	540.79 ± 27.23^g	5.81 ± 0.08^g	5.41 ± 0.34^e	6.80 ± 0.55^h	3.52 ± 0.17^de	8.43 ± 0.14^h	15.49 ± 0.10^f
(E) SA+MOFE 6 mg+300 mg	933.58 ± 27.86^c	8.66 ± 0.49^d	7.39 ± 0.49^c	11.86 ± 0.81^c	3.67 ± 0.10^c	13.18 ± 0.28^c	20.25 ± 0.34^b
(F) SA+MOFE 12 mg+150 mg	707.50 ± 2.54^e	5.70 ± 0.10g^h	5.05 ± 0.15^f	7.35 ± 0.18^g	3.45 ± 0.05^ef	8.90 ± 0.10^g	13.93 ± 0.20^g
(G)SA+MOFE 12 mg+150 mg	773.48 ± 20.81^d	7.25 ± 0.26^e	6.57 ± 0.28^d	9.66 ± 0.31^d	3.57 ± 0.16^cd	12.51 ± 0.28^d	19.30 ± 0.70^c
(H)SA+MOLE 6 mg+150 mg	609.00 ± 1.01^f	5.88 ± 0.11^f	5.06 ± 0.16^f	7.78 ± 0.19^f	3.41 ± 0.03^e	9.73 ± 0.25^f	15.93 ± 0.39^e
(I) SA+MOLE 6 mg+300 mg	1328.43 ± 17.75^a	9.69 ± 0.23^b	8.17 ± 0.43^b	13.03 ± 0.57^b	4.46 ± 0.49^a	15.26 ± 0.46^a	20.42 ± 0.54^b
(J) SA+ MOLE 12 mg+150 mg	718.90 ± 11.12^e	9.29 ± 0.23^f	5.51 ± 0.16^e	8.09 ± 0.03^e	3.65 ± 0.20^c	10.28 ± 0.34^e	16.44 ± 0.78^d
(K)SA+MOLE 12 mg+300 mg	1287.67 ± 20.60^b	9.12 ± 0.67^c	8.14 ± 0.25^b	11.84 ± 0.73^c	4.19 ± 0.17^b	14.50 ± 1.07^b	20.37 ± 1.34^b

[1] *e-mail: asia.iqbal@uvas.edu.pk

Brasil

Brasil

Amelioration potential of Moringa oleifera extracts against sodium arsenate induced embryotoxicity and genotoxicity in mouse (Mus musculus)

Potencial de melhoria de extratos de Moringa oleifera contra embriotoxicidade e genotoxicidade induzida por arseniato de sódio em camundongos (Mus musculus)

Abstract