A Simple and Low-Cost, but Naturalistic Method for Introducing Students to Action Potentials

HIGHLIGHTS

Action potential (AP) in earthworm.

Low-cost amplifier for AP determination.

Big neural cord in earthworm.

Introducing students to neuroscience.

Abstract

Current trends in science education recommend the complementary use of virtual and hands-on methods of teaching. In neurobiology, for instance, there is a plethora of virtual laboratories and simulators that can be readily combined with traditional physical labs. Unfortunately, physical laboratories are almost unaffordable for many institutions due to the high cost of equipment. In this paper, we present a simple and low-cost in vivo method for demonstrating some of the basic biophysical properties of neural action potentials. The method involves the following steps: a) dissection of the ventral nerve cord of earthworm; b) electrical stimulation; c) amplification and visualization of the medial and lateral giant fibers’ action potentials; and d) recording. The system showed stability, expected amplification, high signal-to-noise ratio, and an estimated total cost of US$ 5.662. We provide guidelines for assembling the system and discus its utility as a teaching alternative for low budget institutions.

Keywords:
Action potential; low-cost action potential laboratory; neurobiology lab; equitable science education