Biological and Oxidative Effect of Ellagic Acid on Saccharomyces cerevisiae: A New Way for Culture Developing

HIGHLIGHTS

Ellagic acid (EA) reduces oxidative damage and thus it can protect S. cerevisiae against to ROS.

SDS-PAGE results reveal that EA induces total protein synthesis.

EA induces S. cerevisiae cell development and it reduces MDA levels and it increases GSH levels.

Abstract

In this study, the effects of Ellagic acid (EA) on protein expression in yeasts and cellular development were investigated. Four groups were formed. Groups: 1) Control group; yeast only cultivated group; 2) Ellagic Acid (EA) group: EA (10%) given group; 3) Hydrogen peroxide (H₂O₂) Group: The group given H₂O₂ (15 mM); 4) EA + H₂O₂ group: EA (10%) + H₂O₂ (15 mM) group. After sterilization, EA (10%) and H₂O₂ (15 mM) were added to the Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours (overnight). S. cerevisiae cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) level were analyzed by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and measured by the Bradford method. According to the obtained results, compared with the H₂O₂ group, cell development (1, 3, 5 and 24 hours), GSH level and total protein synthesis (24 hours) were increased with EA, while MDA level (24 hours) decreased. These results show that EA reduces oxidative damage, increases cell growth and it has a protective effect to promote protein synthesis in S. cerevisiae culture.

Keywords:
Ellagic acid; H₂O₂; Saccharomyces cerevisiae; SDS-PAGE

GRAPHICAL ABSTRACT