Quantification of 5-methylcoumarin-4-glucoside and 11,12-dihydroxy-5-methylcoumestan in Six Peruvian Species of the Genus Mutisia (Asteraceae)

Abstract

Mutisia L. f. (Mutisiinae, Mutisieae, Mutisioideae, Asteraceae) is a South American genus that contains several medicinal species. Mutisia acuminata Ruiz & Pav. and M. orbignyana Wedd. are known sources of the 12-dihydroxy-5-methylcoumestan and 5-methylcoumarin-4-glucoside respectively. The 12-dihydroxy-5-methylcoumestan exhibited notable antihepatotoxic activity in a previous study. In the present work, 5-methylcoumarin-4-glucoside and 12-dihydroxy-5-methylcoumestan were isolated from a Peruvian collection of M. orbignyana. Additionally, the relative concentrations of those two isolated compounds in six Peruvian species of Mutisia (M. acuminata, M. cochabambensis Hieron., M. lanata Ruiz & Pav., M. orbignyana, M. venusta S.F. Blake and M. wurdackii Cabrera) were determined by an HPLC method. The highest concentrations of both constituents were observed in the sample of M. orbignyana.

Keywords:
Mutisia; Asteraceae; 5-methylcoumarin; coumestan

HIGHLIGHTS

• A 5-methylcoumarin and a coumestan were isolated from M. orbignyana.

• An HPLC method was established for the quantification of both compounds.

• The concentrations of both compounds were assessed in six samples of Mutisia.

• Mutisia orbignyana displayed the highest contents of both isolated compounds.

GRAPHICAL ABSTRACT