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Agriculture, Agribusiness and Biotechnology • Braz. arch. biol. technol. 47 (1) • Mar 2004 • https://doi.org/10.1590/S1516-89132004000100002   copy

Varietal identification of coffee seeds by RAPD technique

Authorship SCIMAGO INSTITUTIONS RANKINGS

This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.

Coffea; cultivar identification; molecular marker

Instituto de Tecnologia do Paraná - Tecpar Rua Prof. Algacyr Munhoz Mader, 3775 - CIC, 81350-010 Curitiba PR Brazil, Tel.: +55 41 3316-3052/3054, Fax: +55 41 3346-2872 - Curitiba - PR - Brazil
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