Abstract
The purpose of this study was to investigate the antioxidant and antimicrobial activity of Peganum harmala seed extract, as well as to determine its fatty acid profile. The highest zone diameter of P. harmala seed extract formed on S. aureus, E. coli, and Y. enterocolitica was determined to be 30 mm, and the MIC value on these bacteria was 31.2 µg mL-1. It has a high level of antimicrobial effect. Among the four different solvent extracts, the highest absorbance value in the CUPRAC method was observed in water extract (0.714), the highest inhibition value in the DPPH method methanol extract (74.06%) and in the ABTS method (72.06%) ethanol extract. A total of 17 fatty acid components were detected in P. harmala seeds, with the highest proportion of linoleic acid with 61.46%. The P. harmala seed can be a good alternative to drugs in the prevention of microbial resistance.
Keywords:
Antimicrobial; Antioxidant; MIC; GC-FID; Peganum harmala seed
HIGHLIGHTS
• S. aureus, E. coli, Y. enterocolitica bacteria showed the highest antimicrobial effect.
• The antioxidant activities were investigated four different extracts prepared with solvents of different polarities.
• Fatty acid content was analyzed by GC-FID.
• Linoleic acid was the most abundant fatty acid detected.
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