Transgenerational memory of drought stress and low rates of glyphosate reduce the sensitivity of <i>Eragrostis plana</i> to the herbicide

Fipke, Marcus V.; Balbinot, Andrisa; Viana, Vívian E.; Gehrke, Vinicios R.; Kemmerich, Magali; Dayan, Franck E.; Souza, Gustavo M.; Camargo, Edinalvo R.; Avila, Luis A.

doi:10.51694/AdvWeedSci/2022;40:00016

Marcus V. Fipke

conceptualization of the manuscript and development of the methodology

data collection and curation

data analysis

data interpretation

writing the original draft of the manuscript

writing, review and editing

Crop Protection Departament , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0002-3057-9522

Andrisa Balbinot

data collection and curation

data analysis

writing, review and editing

Crop Protection Departament , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0002-2380-7523

Vívian E. Viana

conceptualization of the manuscript and development of the methodology

data analysis

data collection and curation

data interpretation

writing the original draft of the manuscript

writing, review and editing

Crop Protection Departament , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0002-8326-7549

Vinicios R. Gehrke

data collection and curation

data analysis

writing, review and editing

Crop Protection Departament , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0002-8459-9318

Magali Kemmerich

conceptualization of the manuscript and development of the methodology

data collection and curation

data interpretation

writing, review and editing

Federal University of Pampa , Itaqui , RS , Brazil .

http://orcid.org/0000-0002-8374-4106

Franck E. Dayan

data interpretation

writing, review and editing

Agricultural Biology Department , Colorado State University , Fort Collins , CO , USA .

http://orcid.org/0000-0001-6964-2499

Gustavo M. Souza

conceptualization of the manuscript and development of the methodology

data interpretation

writing, review and editing

Botany Department , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0001-6754-4860

Edinalvo R. Camargo

conceptualization of the manuscript and development of the methodology

funding acquisition and resources

writing, review and editing

Crop Protection Departament , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0001-8025-1042

Luis A. Avila ^** Corresponding author: laavilabr@gmail.com

conceptualization of the manuscript and development of the methodology

data interpretation

funding acquisition and resources

supervision

writing, review and editing

Crop Protection Departament , Federal University of Pelotas , Pelotas , RS , Brazil .

http://orcid.org/0000-0002-2532-7152

* Corresponding author: laavilabr@gmail.com

Author’s contributions

All authors read and agreed to the published version of the manuscript. MVF, VEV, MK, GMS, ERC and LAA: conceptualization of the manuscript and development of the methodology. MVF, AB, VEV, VRG and MK: data collection and curation. MVF, AB, VRG and VEV: data analysis. MVF, VEV, MK, FED, GMS, LAA: data interpretation. ERC and LAA: funding acquisition and resources. LAA: supervision. MVF and VEV: writing the original draft of the manuscript. MVF, AB, VEV, VRG, MK, FED, GMS, ERC and LAA: writing, review and editing:

Approved by:

Editor in Chief: Carlos Eduardo Schaedler

Associate Editor: Lauren Lazaro

Conflict of Interest: The authors declare that there is no conflict of interest regarding the publication of this manuscript.

Gene	Forward (5’-3’)	Reverse (5’-3’)	Reference
OsACT1	CCTTCAACACCCCTGCTATG	CAATGCCAGGGAACATAGTG	Zhou et al., 2012Zhou Y, Lu D, Li C, Luo J, Zhu BF, Zhu J et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor Shattering Abortion1 . Plant Cell. 2012;24(3):1034-48. Available from: https://doi.org/10.1105/tpc.111.094383 https://doi.org/10.1105/tpc.111.094383...
Os18S	CTACGTCCCTGCCCTTTGTACA	ACACTTCACCGGACCATTCAA	Jain et al., 2006Jain M, Nijhawan A, Tyagi AK, Khurana JP. Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Comm. 2006;345(2):646-51. Available from: https://doi.org/10.1016/j.bbrc.2006.04.140 https://doi.org/10.1016/j.bbrc.2006.04.1...
OsEF1 α	TTTCACTCTTGGTGTGAAGCAGAT	GACTTCCTTCACGATTTCATCGTAA	Zhou et al., 2012Zhou Y, Lu D, Li C, Luo J, Zhu BF, Zhu J et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor Shattering Abortion1 . Plant Cell. 2012;24(3):1034-48. Available from: https://doi.org/10.1105/tpc.111.094383 https://doi.org/10.1105/tpc.111.094383...
EcAKR	AGGCCGGTTACAGACACATC	CACTACGCCCTCCTGGAATA
EcEPSPS	GACCGATTGGTGACTTGGTT	TCCTCCAATTCCCTTGACAC
EcMRP10	CACAGCATTTGTTGCTCAGACT	GACTTCTGAATATCTCTCCGGTTG
EcMRP8	AGTGGATTTCTTGCATAATGTTGA	GACTGTGCAATCATACCATCTCTC

^aPOP	^bGen	^cEquation parameters (SE)				^dD₅₀	CI 95%	Sensitivity reduction rate

		a	b	R²	P
^eVisual injury
CHK	G ₀	99.0 (1.92)**	37.29 (3.68)**	0.96	<0.01	172.9	(166.2-178.7)	1.00
CHK	G ₁	98.5 (1.57)**	50.59 (3.59)**	0.98	<0.01	228.8	(222.6-234.2)	1.00
DRY	G ₁	95.7 (1.99)**	47.42 (4.47)**	0.96	<0.01	216.2	(207.9-223.3)	0.94
GLY	G ₁	94.8 (2.92)**	67.19 (8.16)**	0.92	<0.01	215.9	(201.8-226.9)	0.94
DRY×GLY	G ₁	101.3 (3.76)**	99.15 (13.07)**	0.91	<0.01	215.3	(185.8-244.7)	0.94
CHK	G ₂	97.2 (3.00)**	102.8 (9.19)**	0.95	<0.01	278.0	(253.1-302.8)	1.00
DRY	G ₂	97.4 (1.50)**	57.24 (3.65)**	0.98	<0.01	223.4	(217.3-228.9)	0.80
GLY	G ₂	100.0 (2.86)**	90.73 (8.42)**	0.94	<0.01	244.1	(231.8-253.7)	0.88
DRY×GLY	G ₂	124.9 (32.50)**	222.06 (47.45)**	0.86	<0.01	443.0	(425.6-462.7)	1.59
Shoot dry weight
CHK	G ₀	100.6 (8.64)**	0.91 (0.22)**	0.60	<0.01	122.4	(55.8-189.0)	1.00
CHK	G ₁	101.4 (6.05)**	1.24 (0.18)**	0.77	<0.01	199.4	(144.0-254.7)	1.00
DRY	G ₁	102.8 (7.10)**	1.51 (0.27)**	0.68	<0.01	273.7	(194.6-352.9)	1.37
GLY	G ₁	101.1 (6.74)**	1.46 (0.26)**	0.70	<0.01	278.9	(198.8-358.9)	1.39
DRY×GLY	G ₁	100.0 (8.06)**	0.97 (0.25)**	0.56	<0.01	251.2	(130.1-372.2)	1.25
CHK	G ₂	99.9 (5.89)**	0.89 (0.55)**	0.73	<0.01	152.5	(94.5-210.5)	1.00
DRY	G ₂	98.8 (6.47)**	2.19 (0.43)^**	0.74	<0.01	283.6	(218.6-348.6)	1.85
GLY	G ₂	95.6 (6.29)**	1.64 (0.30)**	0.72	<0.01	308.5	(226.9-390.2)	2.02
DRY×GLY	G ₂	99.5 (6.41)**	1.31 (0.70)**	0.63	<0.01	435.9	(294.0-577.7)	2.85

[1] * Corresponding author: laavilabr@gmail.com

Brasil

Brasil

Transgenerational memory of drought stress and low rates of glyphosate reduce the sensitivity of Eragrostis plana to the herbicide

Abstract

Background

Objective

Methods

Results

Conclusions