Placenta specific gene targeting to study histone lysine demethylase and androgen signaling in ruminant placenta

Hord, Taylor Kimberly; Aubone, Agata Maria Parsons; Ali, Asghar; Templeton, Hayley Nicole; Evans, River; Bruemmer, Jason Edward; Winger, Quinton Alexander; Bouma, Gerrit Jerry

doi:10.1590/1984-3143-AR2020-0069

Taylor Kimberly Hord

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

Agata Maria Parsons Aubone

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

Asghar Ali

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

Hayley Nicole Templeton

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

River Evans

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

Jason Edward Bruemmer

Animal Reproduction and Biotechnology Laboratory, Department of Animal Sciences, Colorado State University, Fort Collins, CO, USA

Quinton Alexander Winger

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

Gerrit Jerry Bouma ^**Corresponding author: gerrit.bouma@colostate.edu

Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

http://orcid.org/0000-0003-2433-5086

*Corresponding author: gerrit.bouma@colostate.edu

Conflicts of interest: The authors have no conflict of interest to declare.

#
These authors contributed equally to this work.

Author contributions TKH: Conceptualization, Data curation, Visualization, Writing – original draft; AMPA: Conceptualization, Data curation and Writing – original draft, Writing – review & editing; AA: Investigation, Data curation; HNT: Writing – review & editing, Visualization; RE: Writing – review & editing, Visualization; JEB: Writing – review & editing; QAW: Writing – review & editing; GJB: Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing.

	Bovine	Ovine
Gestation Length	279-287 Days	144-152 Days
Placentome Shape	Cotelydonary, convex	Cotelydonary, concave
Average Placentome Amount	75-125	75-125
Blastocyst Hatching	Days 7-10	Days 7-8
Conceptus Growth	Day 15: 1-2 mm Days 18-19: 10-20 cm	Day 12: 10-22 mm Day 14: 10 cm Day 17: 25 cm
Implantation Attachment Time	Beginning Day: 22 Completion Day: 40	Beginning Day: 15-16 Completion Day: 22-28
Total Placentome Weight	• Rate of increase decreases with time, with a significant increase between 60-190 days after conception • Up to 5000 g	Increases throughout pregnancy up to 579.4 g
Average Placentome Weight (g)	50 g	7.91
Total Caruncular Weight (g)		101.6
Total Cotyledonary Weight (g)		291.5
Luteal Progesterone	Required throughout gestation	Placenta takes over production around day 50-60
Binucleate Trophoblast Cells	• Formed from mitotic divisions of mononucleate cells that do not undergo cytokinesis • Produce bovine placental lactogen	• Formed from mitotic divisions of mononucleate cells that do not undergo cytokinesis between days 14-16 • Produce ovine placental lactogen • Express placental lactogens and pregnancy-associated glycoproteins
Trophoblast Giant Cells/Multinucleated Trophoblast Cells	• Involved in syncytialization • Formed through mononuclear trophoblast cell fusion • Migrate and fuse with uterine epithelium forming syncytium • Function in gas and nutrient exchange and hormone production	• Involved in syncytialization • Formed through mononuclear trophoblast cell fusion • Insert between the uterine epithelial cells that are simultaneously undergoing apoptosis • Fuse with each other to form trophoblast syncytial layer • Function in gas and nutrient exchange and hormone production

[1] *Corresponding author: gerrit.bouma@colostate.edu

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Placenta specific gene targeting to study histone lysine demethylase and androgen signaling in ruminant placenta

Abstract