O HLA-DQA1*04:01 está relacionado com uma alta carga lesional na ressonância magnética em T2/Flair nos pacientes com esclerose múltipla

Fabio NORO

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Soniza Vieira ALVES-LEON

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Fabricia Lima FONTES-DANTAS

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Paulo Roberto VALLE BAHIA

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Rodrigo Ferrone ANDREIUOLO

Rede Dor-São Luiz, Rio de Janeiro RJ, Brazil.

Fernanda Cristina RUEDA LOPES

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Valeria Coelho Santa Rita PEREIRA

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Livia de Almeida Afonso ABI-HAILA

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Renan Amaral COUTINHO

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Amanda Dutra de ARAUJO

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Edson MARCHIORI

Universidade Federal do Rio de Janeiro, Departamento de Radiologia, Rio de Janeiro RJ, Brazil.

Authors’ contributions: FN: conceptualization, methodology, validation, formal analysis, investigation, writing of original draft, project administration; SVAL: conceptualization, methodology, writing (review and editing); FLFD: validation, writing (review and editing); PRVB: conceptualization, methodology; RFA, FCRL, VCSRP, LAAAH, RAC, ADA: investigation; EM: writing (review and editing), formal analysis.

Sequences		Matrix	FOV	slice	TR	TE	Flip
Brain	T1 MPR Sag	256×256	250	1	1940	295	15
	DP+T2 TSE Ax	320×126	230	4	3100	7.3	150
	T2 Flair Sag	256×244	230	4	9000	83	180
	T1 SE Ax MT	256×144	230	5	505	9	90
	Flair 3D Sag	256×218	260	1	5000	418	Empty
	Diffusion	160×160	240	5	3500	83	Empty
	T2 TSE Ax	320×216	220	3	3700	102	150
	Epi 2D – DTI	160×160	240	3	4000	82	Empty
	Swi 3D Ax	256×177	230	2	49	40	15
SPINE	T1 TSE Sag Cerv	320×224	220	3	463	9	132
	T1 TSE Sag Dors	512×307	320	35	645	10	150
	Stir Sag Cerv	320×256	250	3	4170	87	150
	Stir Sag Dors	320×224	320	3.5	5120	86	150
	T2 Med2 Ax Dors	320×24	250	4.5	602	18	30
	T2 Med2 Ax Cerv	320×192	200	4	606	18	30
	T2 TSE Sag	320×224	220	3	2940	81	150

	n	Age (±SD) - range	Sex (M/F)	Age at illness onset: mean (±SD)	Mean time of illness (months): mean (±SD)	Age at MRI moment: mean (±SD)	EDSS: mean (±SD)
RR	73	27.9 (10.9) 5–56	24/49	27.9 (10.9)	17.1 (11.4)	45.1 (13.4)	6.8 (2.3)
PP	9	37.6 (7.4) 27–47	7/2	37.6 (7.4)	15.7 (14.8)	54.6 (12.8)	5.1 (1.6)
SP	13	31.9 (12.9) 12–57	4/9	31.9 (12.9)	12 (13.7)	53.1 (16.4)	6.8 (2.3)

SNPs		mLL≥19.8 mL n/(%)	mLL<19.8 mL n/(%)	Total n/%	p-value
rs3087456	AH	40/51	39/49	79/100	0.41
	HZ	3/37	5/35	8/100
	PH	5/71	2/29	7/100
rs4774	AH	2/33	4/67	6/100	0.66
	HZ	8/53	7/47	15/100
	PH	38/52	35/48	73/100
rs6897932	AH	1/100	0/0	1/100	0.61
	HZ	7/50	7/50	14/100
	PH	40/51	39/49	79/100
rs731236	AH	43/51	41/49	84/100	0.94
	HZ	5/50	5/50	10/100
	PH	48/51	46/49	94/100
rs1033182	AH	1/50	1/50	2/100	0.75
	HZ	4/40	6/60	10/100
	PH	43/52	39/48	82/100

	Alleles	Median total adjusted load lesion		Total
	Alleles	≥19.8 mL	<19.8 mL	Total
HLA DQA1 n (%)	01:02	2 (100)	0	2 (100)
	03:01	0	1 (100)	1 (100)
	04:01	17 (71)	7 (29)	24 (100)
	02:01	3 (60)	2 (40)	5 (100)
	05:01	18 (41)	26 (59)	44 (100)
	01:04	1 (25)	3 (75)	4 (100)
	05	0	1 (100)	1 (100)
	05:02	2 (40)	3 (60)	5 (100)
	06:01	0	2 (100)	2 (100)
Patients (total) — n (%)		43 (49)	45 (51)	88 (100)
Chi-square tests		Value	df	p-value
Pearson chi-square		12.982	8	0.112
Likelihood ratio		15.481	8	0.050
Linear-by-linear association		8.285	1	0.004
n of valid cases		88

Brasil