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Cerebrospinal fluid cytomorphology in the normal newborn infant

Total cell count of cerebrospinal fluid (CSF) in normal newborn infants has shown no more than 12 leucocytes and 600 red cells by cubic millimeter, as it was previously reported by one of us5. Results on CSF cytomorphology are reported in this study. CSF cytomorphology was studied in 21 normal newborn infants: 2 days old (17 cases) and 7 days old (4 cases). The samples were collected through lumbar puncture. Cytomorphological examinations were carried out after preparing the samples by accelerated gravitational sedimentation in Suta's chamber; the sediments were stained by Leishman's technique. Cytomorphology was type II in most of the cases. Lymphocytes and reticulomonocytes were found in all cases, the relative proportion being 11-59% and 41-78% respectively. Polinuclear neutrophils were found in 12 cases, and the higher relative proportion observed for them was 17%. Eosinophil cells occurred in 3 cases, in the proportion of no more than 2%. Macrophages occured in 12 cases, and the higher proportion observed was 10%. The average values were: lymphocytes 34,6%, reticulomonocytes 60.4%, polinuclear neutrophils 2.9%, eosinophil cells 0.2%, macrophages 1.9%. Linear cell groupings occurred in 3 cases; their nature was morphologically related to choroid cells. Polymorph groupings of undiferenciated reticular cells were found also. They occurred in 5 cases, and their origin was related to cells of arachnoid membrane.


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