Acessibilidade / Reportar erro

DIAGNOSTIC METHODS FOR THE FOOD PATHOGENS CAMPYLOBACTER SP., SALMONELLA SP. AND LISTERIA MONOCYTOGENES

ABSTRACT

The molecular methods for quick and efficient detection of chicken lots infected by bacteria such as Salmonella sp. Campylobacter sp. and Listeria monoytogenes is basic for the effort to reduce the frequency of the transmission between chicken lots and to the consumers of poultry products. Recently, the development of techniques involving molecular biology, especially polymerase chain reaction, which allows the specific enlargement of segments of DNA, has been making new procedures possible for the identification of the abovementioned bacteria, reducing the time necessary for the tests and enhancing the reliability of the resulting diagnoses. The use of molecular biology in laboratories for human and animal diagnosis, as well as in quality control programs for foods and products of animal origin is already a reality and has tended to expand quickly. The objective of this article is to present a brief review of the conventional diagnostic and molecular tests for the identification of Campylobacter sp., Salmonella sp. and Listeria monocytogenes. In conclusion, molecular diagnosis is a field undergoing scientific and technological advancement, in which new molecular techniques are under development for the diagnosis of bacteria in foods.

KEY WORDS
Diagnosis; chicken; Salmonella sp.; Campylobacter sp.; Listeria monocytogenes

Instituto Biológico Av. Conselheiro Rodrigues Alves, 1252 - Vila Mariana - São Paulo - SP, 04014-002 - São Paulo - SP - Brazil
E-mail: arquivos@biologico.sp.gov.br