ABSTRACT
Caprine Arthritis Encephalitis virus (CAEV) and Visna/maedi virus (VISNA), belong to the genus Lentivirus family Retroviridae. The aim of the present study was to isolate the CAE virus from a positive goat when tested by agarose gel immunodifusion (AGID) by cocultivation techniques of infected peripheral blood leukocytes in goat synovial membrane (GSM). In this study, one flock of 16 goats, obtained from a flock of Rio Grande do Norte, Brazil, with clinical suspicions for CAEV, was screened using agar-gel immunodiffusion (agid) and Western blotting. The result was positive for one animal, confirmed by Western blotting. For virus isolation, peripheral blood leukocytes (PBL) were isolated from the blood for co-culture on goat sinovial membrane (GSM) cells. Monocytes/macrophages collected were inoculated in a monolayer of 90% semiconfluent cells in the A25 culture bottles. After 50 days of co-culture, the monolayer was stained with crystal violet 0.1% for viewing viral cytopathic effects (CPE) characteristic of viruses, and nested-PCR was performed with the supernatant of co-culture for confirmation of CPE. The isolate, named BrRN-CNPC.G1, was considered the first isolation of small ruminant lentivirus from a naturally infected goat in a flock in Rio Grande do Norte, Brazil. This study will soon allow for the molecular characterization of the isolated virus genome, by analysis of its different structural genes and their comparison with other isolated viral sequences to identify the likely source of infection of that animal and establish the possible differences between strains of lentiviruses and circulating regional strains.
KEY WORDS
Co-culture; goat sinovial membrane; CAEV
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