The present research was developed at Dourados, Mato Grosso do Sul State, Brazil, in the agricultural year of 1980-1981 with the aim of evaluating the effect of the incorporation depth of the main herbicides used soybean. There fore two experiments have been conducted, being one in the field and the other in pots at greenhouse . Herbicides have been utilized are trifluralin, metribuzin, vernolate and pendimethalin in respective doses of 1.11, O.36, 3-86 and 1.50 kg a.i./ha incorporated to the soil at depth of 0, 2..5, 5.0 and 10.0 cm, being sown the variety of Paraná soybean in the field experiment, and Bossier, Santa Rosa and Paraná varieties in the greenhouse. The assessment of results has been carried out by collecting data of plants height, initial and final population, and weeds dry matter weigh and production data presented by soybean at different applications, being observed that for field conditions, incorporation depth has not affected soybean development and production, expressed by its dry matter weigh. However, at greenhouse conditions, incorporation depth has affected dry matter weigh in the aerial and radicular part and plants height. Aerial part height and dry matter weigh have been more affected by vernolate when incorporated at 10 cm. Trifluralin incorporated at 10 cm and pendimethalin applied at the surface have affected at a lower intensity as well. The radicular system has been more at fee. led by both pendimethalin and vernolate. The Santa Rosa variety has been the one that showed itself most sensitive to the utilized herbicides.