Caracteres culturais das bactérias dos nódulos de algumas leguminosas tropicais

Caracteres culturais das bactérias dos nódulos de algumas leguminosas tropicais^*

Ferdinando Galli

E. S. A. "Luiz de Queiroz"

SUMMARY

23 isolates from root nodules of 13 leguminous species were tested in laboratory for cultural and physiological characteristics.

The following conclusions can be drawn:

1 - The cultural and physiological characters were not enough to identify the different isolates.

2 - The data from cultural and physiological tests help the identification of the bacteria, when checked with data from cross-inoculation tests reported elsewhere (GALLI, 1958). The bacteria studied herein may be grouped as the following:

a) nodule bacteria from Vigna sinensis, Calopogonium muconoides, Centrosema pubescens, Desmodium adscendens, D. discolor, Indigofera mucronata, I. subulata, I. sumatrana, Stylosanthes guyanensisA Tephrosia cândida and Teramnus uncinatus: bacteria with scanty growth; alcaline reaction in milk-brom cresol purple; no acid production in medium with any of the carbohydrates used.

b) nodule bacteria from pea and from Vicia obscura: bacteria with moderate growth; alcaline reaction, with serum zone, in milk-brom cresol purple; acid production in medium with all the carbohydrates used.

c) nodule bacteria from Leucaena glauca: bacteria with moderate growth; alcaline reaction, with serum zone, in milk-brom cresol purple; acid production from rafinose and manitol.

d) nodule bacteria from Cratylia floribunda: bacteria with scanty growth; alcaline reaction in milk-brom cresol purple; acid production from mannose and ramnose.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

BIBLIOGRAFIA

• ALLEN, O. N., 1951 - Experiments in soil bacteriology. Burgess Publishing Co., Minneapolis. 127 p.
• AQUINO, D. I. & A. L. MADAMBA, 1940 - A study of root nodule bacteria of certain leguminous plants. Phillipine Agric. 28: 120-132.
• BALDWIN, I. L. & E. B. FRED, 1927 - The fermentation characters of the root nodule bacteria of the leguminosae. Soil Sci., 24: 217-230.
• BUSHNELL, O. A. & W. B. SARLES, 1937 - Studies on the root nodule bacteria of wild leguminous plants in Wisconsin. Soil Sci. 44: 409-423.
• FRED, E. B., & S. A. WAKSMAN, 1928 - Laboratory manual of general microbiology. McGraw-Hill Book Co., Inc. New York. 145 p.
• GALLI, F., 1958 - Inoculações cruzadas com bactérias dos nódulos de leguminosas tropicais. Rev. Agric. (Piracicaba) 33: 139-150.
• GREGORY, K. F. & O. N. ALLEN, 1953 - Physiological variation and host plant specificities of rhizobia isolated from Caragana arborescens. L. Can. J. Bot. 31: 730-738.
• HOFER, A. W., 1941 - A characterization of Bacterium radiobacter (Beijerinck and van Delden) Lohnis. J. Bact. 41: 193-244.
• JENSEN, H. L., 1942 - Nitrogen fixation in leguminous plants: 1.. General characters of root-nodule bacteria isolated from species of Medicago and Trifolium in Australia. Linnean Soc. New South Wales, Proc. 67: 98-108.
• RIKER, A. J., MARY M. LYNEIS & S. B. LOCKE, 1941 - Comparative physiology of crown gall, attenuated crown gall, Radiobacter and hairy root bacteria. Phytopat. 31: 964-977.
• SOCIETY OF AMERICAN BACTERIOLOGISTS, 1954 a. - Manual of methods for the pure culture study of bacteria. Leaflet IV - Staining methods. Biotech Publications, Geneva, N. Y. 11.Ş ed. 24 p.
• ----------, 1954 b. - Manual of methods for the pure culture study of macteria. Leaflet V - Routine tests fot the descriptive chart. Biotech Publications, Geneva, N. Y. 13.ª ed. 28 p.
• THORNTON, G. D., 1953 - Soil microbiology (Mimeografado) 21 p.

*

No presente trabalho foram utilizados materiais e instalações doados pelo Conselho Nacional de Pesquisas e pela Fundação Rockefeller.

Datas de Publicação

• Publicação nesta coleção
  10 Set 2012
• Data do Fascículo
  1959