The aim of this paper was to study the effect of different concentrations of 2,4-D on the phosphorus uptake by wheat plants (Triticum aestivum, E.) at the 3 leaves stage and its distribution in the plants. Four experiments were carried out in the greenhouse, with four replicates. In the first experiment the wheat plants were harvested 8 h hours after spraying the herbicide. In the second, third and fourth experiments, after 14, 26 and 50 hours respectively. In each experiment, four levels of 2,4-D, namely, 0, 1000, 2000, and 4000 ppm. and a solution with 32P were applied. The data showed: - The 2,4-D affected the phosphorus uptake and its distribution in the plant. - The four experiments showed a tendency toward 2,4-D stimulation of phosphorus uptake in wheat, which decreased with the passage of time. - The phosphorus translocation to the shoot was, in general, stimulated, but this decreased with time. In the third experiment when the plants were sprayed with 2000and 4000 ppm. 2,4-D, the translocation was reduced with respect to the control plants.